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Table 1 Proteins identified through differential BN gel analysis

From: Comparative 1D Blue-Native electrophoresis analysis of Plasmodium falciparum and human proteins associated with cytoadherence

 

Human

 

Parasite

 

Band 1

P50990

T-complex protein 1 subunit theta

PFIT_0712500

Calmodulin

 

P23396

40S ribosomal protein S3

PFIT_1211500

Ca2+-dependent ATPase

 

Q13409

Cytoplasmic dynein 1 intermediate chain 2

  

Band 2

P04264

Keratin, type II cytoskeletal 1

PFIT_0216100

Autophagy-related protein 11, putative

 

P13645

Keratin, type I cytoskeletal 10

 PFIT_1321500

Translation initiation factor EIF-2B subunit related

 

P17661

Desmin

  
 

P35527

Keratin, type I cytoskeletal 9

  
 

A6NNL0

NUT family member 2B

  

Band 3

P17661

Desmin

PFIT_1235400

ATP synthase subunit beta, mitochondrial

 

P13645

Keratin, type I cytoskeletal 10

PFIT_0703500

E3 ubiquitin-protein ligase

 

Q92630

Dual specificity tyrosine-phosphorylation-regulated kinase 2

PFIT_1247800

Unknown function

 

Q92545

Transmembrane protein 131

PFIT_0303600

Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex

 

Q86YH2

Zinc finger protein 280B

  

Band 4

P04075

Fructose-bisphosphate aldolase A

PFIT_1235400

ATP synthase subunit beta, mitochondrial

 

Q14203

Dynactin subunit 1

PFIT_1403800

Unknown function—RBP homology

 

P21796

Voltage-dependent anion-selective channel protein 1

PFIT_0529300

Acyl-CoA synthetase

 

Q13242

Serine/arginine-rich splicing factor 9

PFIT_1032700

Unknown function

Band 5

P21796

Voltage-dependent anion-selective channel protein 1

PFIT_1415500

Atypical protein kinase, ABC-1 family, putative

 

Q8IVL1

Neuron navigator 2

PFIT_1025500

Unknown function

 

Q9GZL7

Ribosome biogenesis protein WDR12

  

Band 6

A5A3E0

POTE ankyrin domain family member F

PFIT_0712500

Calmodulin, putative

 

P40939

Trifunctional enzyme subunit alpha, mitochondrial

PFIT_0533600

Unknown function—Ser/Thr kinase/Arginase

 

Q6ZSB9

Zinc finger and BTB domain-containing protein 49

PFIT_1415500

Atypical protein kinase, ABC-1 family, putative

   

PFIT_1017700

Serine/threonine protein phosphatase 8, putative

   

PFIT_1400200

Erythrocyte membrane protein 1, PfEMP1

Band 7

P00915

Carbonic anhydrase 1

PFIT_0514400

Aspartate–tRNA ligase, putative

 

P35527

Keratin, type I cytoskeletal 9

  
 

Q96T23

Remodeling and spacing factor 1

  
 

Q008S8

Epithelial cell-transforming sequence 2 oncogene-like

  
 

O43506

Disintegrin and metalloproteinase domain-containing protein 20

  
 

Q8TE49

OTU domain-containing protein 7A

  

Band 8

P00915

Carbonic anhydrase 1

PFIT_1022600

Dynein heavy chain, putative

 

P04075

Fructose-bisphosphate aldolase A

PFIT_0421800

Unknown function—Arginase?

 

P04264

Keratin, type II cytoskeletal 1

  
 

P15311

Ezrin

  

Band 9

P00915

Carbonic anhydrase 1

PFIT_1006100

Unknown function

 

P04075

Fructose-bisphosphate aldolase A

PFIT_0809500

Kinesin-like protein, putative

 

P14406

Cytochrome c oxidase subunit 7A2, mitochondrial

  
 

P01138

Beta-nerve growth factor

  

Band 10

P00915

Carbonic anhydrase 1

PFIT_1006100

Unknown function

 

P60709

Actin, cytoplasmic 1

PFIT_0533600

Unknown function—Ser/Thr kinase/Arginase

 

Q12849

G-rich sequence factor 1

PFIT_1135000

Unknown function

 

Q13277

Syntaxin-3

PFIT_1365300

Unknown function—Arginase?

   

PFIT_1033700

Thioredoxin-like associated protein 2, putative

Band 11

Q06830

Peroxiredoxin-1

PFIT_1364900

Ubiquitin-60S ribosomal protein L40

 

P62979

Ubiquitin-40S ribosomal protein S27a

PFIT_1415500

Atypical protein kinase, ABC-1 family, putative

 

P35030

Trypsin-3

PFIT_0516200

Cation-transporting ATPase 1

 

Q96RS6

NudC domain-containing protein 1

PFIT_1310300

Unknown function—nuclear structural protein?

 

Q3V6T2

Girdin

PFIT_1359000

Unknown function—Pf-SET1 homology

 

Q15075

Early endosome antigen 1

  
 

Q7Z429

Protein lifeguard 1

  

Band 12

P13645

Keratin, type I cytoskeletal 10

PFIT_1201800

Unknown function—protein kinase?

 

P35908

Keratin, type II cytoskeletal 2 epidermal

PFIT_1022600

Dynein heavy chain, putative

 

Q8TCT9

Minor histocompatibility antigen H13

  
 

Q13277

Syntaxin-3

  

Band 13

P00338

l-lactate dehydrogenase A chain

PFIT_1222000

Endoplasmin, putative

 

P32119

Peroxiredoxin-2

PFIT_0709100

Unknown function

 

Q9UFH2

Dynein heavy chain 17, axonemal

  
 

P27824

Calnexin

  
 

P04264

Keratin, type II cytoskeletal 1

  

Band 14

P14618

Pyruvate kinase PKM

PFIT_bin02200

A4varTres-erythrocyte membrane protein 1, putative

 

Q13277

Syntaxin-3

PFIT_1033700

Thioredoxin-like associated protein 2, putative

Band 15

Q9Y4L1

Hypoxia up-regulated protein 1

PFIT_0821800

Heat shock protein 70

 

P11142

Heat shock cognate 71 kDa protein

PFIT_1222000

Endoplasmin, putative

 

P13645

Keratin, type I cytoskeletal 10

  
 

Q6UXS9

Inactive caspase-12

  
 

Q13277

Syntaxin-3

  
 

Q9HD43

Receptor-type tyrosine-protein phosphatase H

  

Band 16

Q5T0J7

Testis-expressed protein 35

  

Band 17

Q9Y250

Leucine zipper putative tumor suppressor 1

PFIT_0312000

E3 ubiquitin-protein ligase, putative

 

P32119

Peroxiredoxin-2

  

Band 18

P37802

Transgelin-2

PFIT_0821800

Heat shock protein 70

 

P16520

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

PFIT_1015800

Glycophorin binding protein

 

P61604

10 kDa heat shock protein, mitochondrial

PFIT_1364900

Ubiquitin-60S ribosomal protein L40

 

P40926

Malate dehydrogenase, mitochondrial

PFIT_1424700

Unknown function—Arginase?

 

Q96KP4

Cytosolic non-specific dipeptidase

PFIT_1366800

Secreted ookinete protein, putative—fibrinogen receptor homology

 

P62979

Ubiquitin-40S ribosomal protein S27a

  
 

Q6ZU64

Cilia- and flagella-associated protein 65

  

Band 19

Q99536

Synaptic vesicle membrane protein VAT-1 homolog

PFIT_0108600

Selenocysteine-specific elongation factor selB homologue, putative

 

A6NCI8

Uncharacterized protein C2orf78

PFIT_1108600

Transcription factor with AP2 domain(s)

 

P30485

HLA class I histocompatibility antigen, B-47 alpha chain

  

Band 20

P38646

Stress-70 protein, mitochondrial

PFIT_1324100

l-lactate dehydrogenase

 

P35527

Keratin, type I cytoskeletal 9

PFIT_1364900

Ubiquitin-60S ribosomal protein L40

 

Q9HDC9

Adipocyte plasma membrane-associated protein

PFIT_1246400

Actin I

 

Q8IXQ4

GPALPP motifs-containing protein 1

PFIT_0821800

Heat shock protein 70

 

Q8TF05

Serine/threonine-protein phosphatase 4 regulatory subunit 1

PFIT_1356100

Elongation factor 1-alpha

 

Q9Y3P9

Rab GTPase-activating protein 1

PFIT_1416700

Serine C-palmitoyltransferase, putative

 

Q9H0V9

VIP36-like protein

PFIT_0930800

Peptidyl-prolyl cis–trans isomerase

 

Q9NR99

Matrix-remodeling-associated protein 5

PFIT_0304700

Unknown function

Band 21

Q15084

Protein disulfide-isomerase A6

PFIT_0834600

Heat shock protein 70

 

P38646

Stress-70 protein, mitochondrial

PFIT_1324100

l-lactate dehydrogenase

 

Q96PU4

E3 ubiquitin-protein ligase UHRF2

  
 

P11465

Pregnancy-specific beta-1-glycoprotein 2

  
 

Q9H6F5

Coiled-coil domain-containing protein 86

  

Band 22

Q9HDC9

Adipocyte plasma membrane-associated protein

PFIT_0707300

Heat shock protein 90

 

Q8TF05

Serine/threonine-protein phosphatase 4 regulatory subunit 1

PFIT_0533600

Unknown function—Ser/Thr kinase? Arginase?

 

Q9Y6N3

Calcium-activated chloride channel regulator family member 3

PFIT_0821800

Heat shock protein 70

 

Q6ZTB9

Putative zinc finger protein 833

PFIT_0910700

DNA repair protein REV1, putative

   

PFIT_1246400

Actin I

Band 23

P30101

Protein disulfide-isomerase A3

PFIT_1246400

Actin I

 

P32119

Peroxiredoxin-2

PFIT_0913400

Unknown function—histone modification?

   

PFIT_1206600

Unknown function

   

PFIT_0513300

Unknown function—RBP homology/histone modification?

   

PFIT_0410400

Unknown function—Ser/Thr kinase?

Band 24

P51148

Ras-related protein Rab-5C

PFIT_0617200

60S ribosomal protein L27a, putative

 

Q9P2D7

Dynein heavy chain 1, axonemal

PFIT_0902700

Serine/threonine protein kinase, FIKK family

 

Q9UBM7

7-dehydrocholesterol reductase

PFIT_1359000

Unknown function—Pf-SET1 homology

 

Q8TF05

Serine/threonine-protein phosphatase 4 regulatory subunit 1

PFIT_1252300

Rhoptry neck protein 3

 

Q96PU4

E3 ubiquitin-protein ligase UHRF2

  
 

P02750

Leucine-rich alpha-2-glycoprotein

  

Band 25

P35030

Trypsin-3

PFIT_1356100

Elongation factor 1-alpha

 

O95057

GTP-binding protein Di-Ras1

PFIT_0821800

Heat shock protein 70

   

PFIT_1017700

Serine/threonine protein phosphatase 8, putative

   

PFIT_1246900

Unknown function—Ser/Thr kinase?

   

PFIT_0700800

Unknown function—Ser/Thr kinase?

Band 26

P35527

Keratin, type I cytoskeletal 9

  
 

O43852

Calumenin

  
 

P35030

Trypsin-3

  

Band 27

P27797

Calreticulin

PFIT_1319900

Unknown function—Ser/Thr kinase?

 

Q6NXR4

TELO2-interacting protein 2

  
 

O43572

A-kinase anchor protein 10, mitochondrial

  
 

O95696

Bromodomain-containing protein 1

  
 

Q8IWY8

Zinc finger and SCAN domain-containing protein 29

  
 

P55786

Puromycin-sensitive aminopeptidase

  
 

Q8TF05

Serine/threonine-protein phosphatase 4 regulatory subunit 1

  

Band 28

P68371

Tubulin beta-4B chain

PFIT_1246400

Actin I

 

P27797

Calreticulin

PFIT_1008200

Tubulin beta chain

 

O95057

GTP-binding protein Di-Ras1

PFIT_0533600

Unknown function—Ser/Thr kinase/Arginase?

   

PFIT_1469200

Unknown function – Ser/Thr kinase?

   

PFIT_0317700

CPSF (cleavage and polyadenylation specific factor), subunit A, putative

   

PFIT_0709100

Unknown function

   

PFIT_1350900

Inner membrane complex protein 1f, putative

Band 29

Q8IUR0

Trafficking protein particle complex subunit 5

PFIT_0930800

peptidyl-prolyl cis–trans isomerase

 

P68371

Tubulin beta-4B chain

PFIT_0628900

CPW-WPC family protein

 

Q66K74

Microtubule-associated protein 1S

PFIT_1475300

Unknown function

 

Q9H013

Disintegrin and metalloproteinase domain-containing protein 19

PFIT_1246400

Actin I

Band 30

P37802

Transgelin-2

PFIT_1246400

Actin I

 

P09417

Dihydropteridine reductase

PFIT_1133900

Unknown function

 

P17568

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

PFIT_1350900

Inner membrane complex protein 1f, putative

 

P19827

Inter-alpha-trypsin inhibitor heavy chain H1

PFIT_1329100

Unknown function—Tyr kinase/Ser/Thr kinase?

Band 31

  

PFIT_0621900

Chorismate synthase

Band 32

Q13698

Voltage-dependent L-type calcium channel subunit alpha-1S

PFIT_0821100

14-3-3 protein

 

Q8NBS9

Thioredoxin domain-containing protein 5

PFIT_1123800

Dynein heavy chain, putative

   

PFIT_1229500

Myosin D

  1. Summary table showing differentially identified proteins between binding and non-binding parasite lines from the BN gel slices for human and parasite (IT) components. The sequence identifier and a short description are given for each protein