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Table 1 Proteins identified through differential BN gel analysis

From: Comparative 1D Blue-Native electrophoresis analysis of Plasmodium falciparum and human proteins associated with cytoadherence

  Human   Parasite  
Band 1 P50990 T-complex protein 1 subunit theta PFIT_0712500 Calmodulin
  P23396 40S ribosomal protein S3 PFIT_1211500 Ca2+-dependent ATPase
  Q13409 Cytoplasmic dynein 1 intermediate chain 2   
Band 2 P04264 Keratin, type II cytoskeletal 1 PFIT_0216100 Autophagy-related protein 11, putative
  P13645 Keratin, type I cytoskeletal 10  PFIT_1321500 Translation initiation factor EIF-2B subunit related
  P17661 Desmin   
  P35527 Keratin, type I cytoskeletal 9   
  A6NNL0 NUT family member 2B   
Band 3 P17661 Desmin PFIT_1235400 ATP synthase subunit beta, mitochondrial
  P13645 Keratin, type I cytoskeletal 10 PFIT_0703500 E3 ubiquitin-protein ligase
  Q92630 Dual specificity tyrosine-phosphorylation-regulated kinase 2 PFIT_1247800 Unknown function
  Q92545 Transmembrane protein 131 PFIT_0303600 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex
  Q86YH2 Zinc finger protein 280B   
Band 4 P04075 Fructose-bisphosphate aldolase A PFIT_1235400 ATP synthase subunit beta, mitochondrial
  Q14203 Dynactin subunit 1 PFIT_1403800 Unknown function—RBP homology
  P21796 Voltage-dependent anion-selective channel protein 1 PFIT_0529300 Acyl-CoA synthetase
  Q13242 Serine/arginine-rich splicing factor 9 PFIT_1032700 Unknown function
Band 5 P21796 Voltage-dependent anion-selective channel protein 1 PFIT_1415500 Atypical protein kinase, ABC-1 family, putative
  Q8IVL1 Neuron navigator 2 PFIT_1025500 Unknown function
  Q9GZL7 Ribosome biogenesis protein WDR12   
Band 6 A5A3E0 POTE ankyrin domain family member F PFIT_0712500 Calmodulin, putative
  P40939 Trifunctional enzyme subunit alpha, mitochondrial PFIT_0533600 Unknown function—Ser/Thr kinase/Arginase
  Q6ZSB9 Zinc finger and BTB domain-containing protein 49 PFIT_1415500 Atypical protein kinase, ABC-1 family, putative
    PFIT_1017700 Serine/threonine protein phosphatase 8, putative
    PFIT_1400200 Erythrocyte membrane protein 1, PfEMP1
Band 7 P00915 Carbonic anhydrase 1 PFIT_0514400 Aspartate–tRNA ligase, putative
  P35527 Keratin, type I cytoskeletal 9   
  Q96T23 Remodeling and spacing factor 1   
  Q008S8 Epithelial cell-transforming sequence 2 oncogene-like   
  O43506 Disintegrin and metalloproteinase domain-containing protein 20   
  Q8TE49 OTU domain-containing protein 7A   
Band 8 P00915 Carbonic anhydrase 1 PFIT_1022600 Dynein heavy chain, putative
  P04075 Fructose-bisphosphate aldolase A PFIT_0421800 Unknown function—Arginase?
  P04264 Keratin, type II cytoskeletal 1   
  P15311 Ezrin   
Band 9 P00915 Carbonic anhydrase 1 PFIT_1006100 Unknown function
  P04075 Fructose-bisphosphate aldolase A PFIT_0809500 Kinesin-like protein, putative
  P14406 Cytochrome c oxidase subunit 7A2, mitochondrial   
  P01138 Beta-nerve growth factor   
Band 10 P00915 Carbonic anhydrase 1 PFIT_1006100 Unknown function
  P60709 Actin, cytoplasmic 1 PFIT_0533600 Unknown function—Ser/Thr kinase/Arginase
  Q12849 G-rich sequence factor 1 PFIT_1135000 Unknown function
  Q13277 Syntaxin-3 PFIT_1365300 Unknown function—Arginase?
    PFIT_1033700 Thioredoxin-like associated protein 2, putative
Band 11 Q06830 Peroxiredoxin-1 PFIT_1364900 Ubiquitin-60S ribosomal protein L40
  P62979 Ubiquitin-40S ribosomal protein S27a PFIT_1415500 Atypical protein kinase, ABC-1 family, putative
  P35030 Trypsin-3 PFIT_0516200 Cation-transporting ATPase 1
  Q96RS6 NudC domain-containing protein 1 PFIT_1310300 Unknown function—nuclear structural protein?
  Q3V6T2 Girdin PFIT_1359000 Unknown function—Pf-SET1 homology
  Q15075 Early endosome antigen 1   
  Q7Z429 Protein lifeguard 1   
Band 12 P13645 Keratin, type I cytoskeletal 10 PFIT_1201800 Unknown function—protein kinase?
  P35908 Keratin, type II cytoskeletal 2 epidermal PFIT_1022600 Dynein heavy chain, putative
  Q8TCT9 Minor histocompatibility antigen H13   
  Q13277 Syntaxin-3   
Band 13 P00338 l-lactate dehydrogenase A chain PFIT_1222000 Endoplasmin, putative
  P32119 Peroxiredoxin-2 PFIT_0709100 Unknown function
  Q9UFH2 Dynein heavy chain 17, axonemal   
  P27824 Calnexin   
  P04264 Keratin, type II cytoskeletal 1   
Band 14 P14618 Pyruvate kinase PKM PFIT_bin02200 A4varTres-erythrocyte membrane protein 1, putative
  Q13277 Syntaxin-3 PFIT_1033700 Thioredoxin-like associated protein 2, putative
Band 15 Q9Y4L1 Hypoxia up-regulated protein 1 PFIT_0821800 Heat shock protein 70
  P11142 Heat shock cognate 71 kDa protein PFIT_1222000 Endoplasmin, putative
  P13645 Keratin, type I cytoskeletal 10   
  Q6UXS9 Inactive caspase-12   
  Q13277 Syntaxin-3   
  Q9HD43 Receptor-type tyrosine-protein phosphatase H   
Band 16 Q5T0J7 Testis-expressed protein 35   
Band 17 Q9Y250 Leucine zipper putative tumor suppressor 1 PFIT_0312000 E3 ubiquitin-protein ligase, putative
  P32119 Peroxiredoxin-2   
Band 18 P37802 Transgelin-2 PFIT_0821800 Heat shock protein 70
  P16520 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 PFIT_1015800 Glycophorin binding protein
  P61604 10 kDa heat shock protein, mitochondrial PFIT_1364900 Ubiquitin-60S ribosomal protein L40
  P40926 Malate dehydrogenase, mitochondrial PFIT_1424700 Unknown function—Arginase?
  Q96KP4 Cytosolic non-specific dipeptidase PFIT_1366800 Secreted ookinete protein, putative—fibrinogen receptor homology
  P62979 Ubiquitin-40S ribosomal protein S27a   
  Q6ZU64 Cilia- and flagella-associated protein 65   
Band 19 Q99536 Synaptic vesicle membrane protein VAT-1 homolog PFIT_0108600 Selenocysteine-specific elongation factor selB homologue, putative
  A6NCI8 Uncharacterized protein C2orf78 PFIT_1108600 Transcription factor with AP2 domain(s)
  P30485 HLA class I histocompatibility antigen, B-47 alpha chain   
Band 20 P38646 Stress-70 protein, mitochondrial PFIT_1324100 l-lactate dehydrogenase
  P35527 Keratin, type I cytoskeletal 9 PFIT_1364900 Ubiquitin-60S ribosomal protein L40
  Q9HDC9 Adipocyte plasma membrane-associated protein PFIT_1246400 Actin I
  Q8IXQ4 GPALPP motifs-containing protein 1 PFIT_0821800 Heat shock protein 70
  Q8TF05 Serine/threonine-protein phosphatase 4 regulatory subunit 1 PFIT_1356100 Elongation factor 1-alpha
  Q9Y3P9 Rab GTPase-activating protein 1 PFIT_1416700 Serine C-palmitoyltransferase, putative
  Q9H0V9 VIP36-like protein PFIT_0930800 Peptidyl-prolyl cis–trans isomerase
  Q9NR99 Matrix-remodeling-associated protein 5 PFIT_0304700 Unknown function
Band 21 Q15084 Protein disulfide-isomerase A6 PFIT_0834600 Heat shock protein 70
  P38646 Stress-70 protein, mitochondrial PFIT_1324100 l-lactate dehydrogenase
  Q96PU4 E3 ubiquitin-protein ligase UHRF2   
  P11465 Pregnancy-specific beta-1-glycoprotein 2   
  Q9H6F5 Coiled-coil domain-containing protein 86   
Band 22 Q9HDC9 Adipocyte plasma membrane-associated protein PFIT_0707300 Heat shock protein 90
  Q8TF05 Serine/threonine-protein phosphatase 4 regulatory subunit 1 PFIT_0533600 Unknown function—Ser/Thr kinase? Arginase?
  Q9Y6N3 Calcium-activated chloride channel regulator family member 3 PFIT_0821800 Heat shock protein 70
  Q6ZTB9 Putative zinc finger protein 833 PFIT_0910700 DNA repair protein REV1, putative
    PFIT_1246400 Actin I
Band 23 P30101 Protein disulfide-isomerase A3 PFIT_1246400 Actin I
  P32119 Peroxiredoxin-2 PFIT_0913400 Unknown function—histone modification?
    PFIT_1206600 Unknown function
    PFIT_0513300 Unknown function—RBP homology/histone modification?
    PFIT_0410400 Unknown function—Ser/Thr kinase?
Band 24 P51148 Ras-related protein Rab-5C PFIT_0617200 60S ribosomal protein L27a, putative
  Q9P2D7 Dynein heavy chain 1, axonemal PFIT_0902700 Serine/threonine protein kinase, FIKK family
  Q9UBM7 7-dehydrocholesterol reductase PFIT_1359000 Unknown function—Pf-SET1 homology
  Q8TF05 Serine/threonine-protein phosphatase 4 regulatory subunit 1 PFIT_1252300 Rhoptry neck protein 3
  Q96PU4 E3 ubiquitin-protein ligase UHRF2   
  P02750 Leucine-rich alpha-2-glycoprotein   
Band 25 P35030 Trypsin-3 PFIT_1356100 Elongation factor 1-alpha
  O95057 GTP-binding protein Di-Ras1 PFIT_0821800 Heat shock protein 70
    PFIT_1017700 Serine/threonine protein phosphatase 8, putative
    PFIT_1246900 Unknown function—Ser/Thr kinase?
    PFIT_0700800 Unknown function—Ser/Thr kinase?
Band 26 P35527 Keratin, type I cytoskeletal 9   
  O43852 Calumenin   
  P35030 Trypsin-3   
Band 27 P27797 Calreticulin PFIT_1319900 Unknown function—Ser/Thr kinase?
  Q6NXR4 TELO2-interacting protein 2   
  O43572 A-kinase anchor protein 10, mitochondrial   
  O95696 Bromodomain-containing protein 1   
  Q8IWY8 Zinc finger and SCAN domain-containing protein 29   
  P55786 Puromycin-sensitive aminopeptidase   
  Q8TF05 Serine/threonine-protein phosphatase 4 regulatory subunit 1   
Band 28 P68371 Tubulin beta-4B chain PFIT_1246400 Actin I
  P27797 Calreticulin PFIT_1008200 Tubulin beta chain
  O95057 GTP-binding protein Di-Ras1 PFIT_0533600 Unknown function—Ser/Thr kinase/Arginase?
    PFIT_1469200 Unknown function – Ser/Thr kinase?
    PFIT_0317700 CPSF (cleavage and polyadenylation specific factor), subunit A, putative
    PFIT_0709100 Unknown function
    PFIT_1350900 Inner membrane complex protein 1f, putative
Band 29 Q8IUR0 Trafficking protein particle complex subunit 5 PFIT_0930800 peptidyl-prolyl cis–trans isomerase
  P68371 Tubulin beta-4B chain PFIT_0628900 CPW-WPC family protein
  Q66K74 Microtubule-associated protein 1S PFIT_1475300 Unknown function
  Q9H013 Disintegrin and metalloproteinase domain-containing protein 19 PFIT_1246400 Actin I
Band 30 P37802 Transgelin-2 PFIT_1246400 Actin I
  P09417 Dihydropteridine reductase PFIT_1133900 Unknown function
  P17568 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 PFIT_1350900 Inner membrane complex protein 1f, putative
  P19827 Inter-alpha-trypsin inhibitor heavy chain H1 PFIT_1329100 Unknown function—Tyr kinase/Ser/Thr kinase?
Band 31    PFIT_0621900 Chorismate synthase
Band 32 Q13698 Voltage-dependent L-type calcium channel subunit alpha-1S PFIT_0821100 14-3-3 protein
  Q8NBS9 Thioredoxin domain-containing protein 5 PFIT_1123800 Dynein heavy chain, putative
    PFIT_1229500 Myosin D
  1. Summary table showing differentially identified proteins between binding and non-binding parasite lines from the BN gel slices for human and parasite (IT) components. The sequence identifier and a short description are given for each protein