Fig. 1
Screening of mAbs that are specific to the EWGWS epitope in Pfeno. a Reactivity of mAbs with WT-rPfeno and Δ5-rPfeno as measured by ELISA. Reactivity ratio (green bars) is defined as the ratio of the two reactivities, i.e., O.D.WT-rPfeno/O.D. Δ5-rPfeno. b Reactivity of anti-rPfeno monoclonal antibodies with yeast enolase that lacks the insert sequence. Three mAbs with high reactivity ratio namely H12E1, H12B8 and B1B3 were used in this ELISA. c Western blot analysis of cell lysates from different organisms (P. yoelii, P. berghei, Toxoplasma gondii, Brassica spp., Arabidopsis thaliana, Human Embryonic Kidney cells (HEK 293T) and ∆5-rPfeno). Blot was probed using anti-rPfeno mAb H12E1. Antibody reactivity is observed only for the enolases containing the EWGWS motif. Enolases lacking this insert (HEK 293T and Δ5-rPfeno) did not show any reactivity