Fig. 3

Growth inhibitory activity (GIA) of anti-Pfeno antibodies. a Effect of polyclonal anti-rPfeno sera and mAb H12E1 hybridoma supernatant on in vitro growth of P. falciparum 3D7. ‘Pre-immune (PI) serum’ and ‘no serum’ samples served as controls. Cultures were allowed to grow for 48 h before determining the % parasitemia. Initial parasitemia was 0.5%. b Effect of purified IgGs on parasite growth. Initial parasitemia was 3.2%. % parasitemia was determined at 24 (blue bars) and 48 h (green and red bars). At the 24-h time point all the samples had only schizonts. At 48 h, no IgG and pre-immune IgGs treated samples (contols) showed only ring stage parasites (green bars). Samples treated with polyclonal anti-rPfeno and H12E1 IgGs had rings (green bars) as well as schizonts (red bars) in parasitized cells. c % growth inhibition by different IgGs. Significance of decrease in parasite growth in antibody treated samples was compared with control samples using Student’s t-test. *p < 0.05, **p < 0.01 and ***p < 0.001