Fig. 3

Phylogenetic and species delimitation analysis detailing collection specimens and reference sequences belonging to the Genus Nyssorhynchus. a Consensus tree from a Bayesian phylogenetic analysis using the COI gene. Numbers at branches indicate Bayesian posterior probability (≥ 70%). Haplotypes from collection specimens are coloured: Red = Acre, Green = Amazonas, Blue = Rondônia. Chagasia bonneae was included as an outgroup taxon, b Multi-rate Poisson Tree Process (mPTP) species delimitation analysis, c Automatic Barcode Gap Discovery (ABGD) analysis