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Table 2 PCR results from different Plasmodium malariae concentrations of blood clot and whole blood samples from an infected Alouatta g. clamitans (M3) monkey

From: Frozen blood clots can be used for the diagnosis of distinct Plasmodium species in man and non-human primates from the Brazilian Atlantic Forest

M3 Alouatta g. clamitans

Dilutions

Conditions

Sample

1

1:2

1:4

1:10

1:100

1:1000

1:10,000

1:1,000,000

Regular PCRa conditions

Blood clotb

+

+

+

+

Whole blood

+

+

+

+

+

Improved PCRa conditions

Blood clotb

+

+

+

+

+

Whole blood

+

+

+

+

+

+

  1. aThe gene target was ssrRNA; b DNA extractions from clots disrupted by high-speed shaking (6000 rpm/40 s) in lysis buffer, without glass beads; + and −: positive and negative result, respectively