Strategy to improve malaria surveillance system preventing transfusion-transmitted malaria in blood banks using molecular diagnostic

Table 1 Baseline characteristics of the samples collected in blood banks of the Brazilian Amazon region and exposure to P. vivax and P. falciparum detected by molecular diagnosis

Blood banks located in Pará and Rondônia state	N	Age in years, median (range)	Males, %	API^a	mt-qPCR positive N (%)
Blood banks located in Pará and Rondônia state	N	Age in years, median (range)	Males, %	API^a	P. falciparum	P. vivax
Belém–Pará^b	120	32 (18–59)	85.0	0.7 (low)	0 (0)	0 (0)
Castanhal–Pará	161	31 (19–59)	73.3	0.4 (low)	0 (0)	0 (0)
Marabá–Pará	193	30 (18–65)	75.6	5.9 (low)	0 (0)	0 (0)
Santarém–Pará	197	34 (20–60)	66.0	3.1 (low)	0 (0)	3 (1.5)
Abaetetuba–Pará	167	30 (19–62)	76.0	0.9 (low)	0 (0)	5 (3.0)
Redenção–Pará	129	34 (20–58)	71.3	0.15 (low)	0 (0)	0 (0)
Capanema–Pará	155	28 (19–60)	66.4	0.5 (low)	0 (0)	0 (0)
Tucuruí–Pará	33	32 (18–65)	60.6	35.6 (medium)	0 (0)	0 (0)
Altamira–Pará	169	26 (18–59)	75.7	22.7 (medium)	0 (0)	0 (0)
Porto Velho–Rondônia^c	900	NA	NA	54.3 (high)	0 (0)	2 (0.2)
All samples	2224	30 (18–65)	73.1	–	0 (0)	10 (0.45)

NA not available
^aAnnual Parasite Index at the year of the survey with the respective classifications (low, medium or high) as reported by the Brazilian Ministry of Health (http://www.saude.gov.br/sivep_malaria)
^bAll donors from Pará state were selected to donation
^cAmong all samples collected in Rondônia state, only two were positive for P. vivax by molecular screening, 700 were approved as donors and 200 were excluded, all of them were tested in both molecular methods, the nested polymerase chain reaction (nested-PCR) for amplification of the 18S RNA and the real time PCR (qPCR) to amplify the parasites mtDNA

ISSN: 1475-2875