- The MS individual fragment lengths are shown for 3D7 and E5 as well as for the in silico 3D7 microsatellite lengths in the NCBI database. MS alleles with > 3 bp size difference between 3D7 and E5 are typed as non-3D7 (grey). 3D7 alleles are white, non-3D7 alleles are grey. 3D7 var gene amplification on E5 DNA was verified by targeted Sanger sequencing of PCR fragments. Note that the table differs from Table 1 in Frank et al. [7] at the 5′ end of Chromosome 7 (reannotation of PF3D7_0700100 previously Mal8P1.220) and at the 3′ end of Chromosome 13: PF3D7_1373500 (previously MAL13P1.356)