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Table 1 Malaria diagnostic strategies used to classify the presence or absence of malaria parasites among the U5 children in the study

From: Can malaria rapid diagnostic tests by drug sellers under field conditions classify children 5 years old or less with or without Plasmodium falciparum malaria? Comparison with nested PCR analysis

No. Sample/other information Diagnostic strategy Remarks
1 Finger prick blood on RDT strip Drug seller interpretation of RDT strip (drug seller) Diagnostic test data obtained from drug shop patient registry
2 Retrieved RDT strip at central lab Laboratory scientist repeat reading of malaria RDT strip (laboratory scientist) First comparator for drug seller interpretation of the RDT strip
3 P. falciparum DNA extracted from the RDT nitrocellulose strip PCR analysis of P falciparum DNA (RDT-PCR) Used malaria RDT cassettes were transferred from the field to the molecular laboratory at Makerere University College of Health Sciences in Kampala
4 P. falciparum DNA extracted from dried blood spot on FTA filter paper PCR analysis of P falciparum DNA (FTA-PCR) Second reference and gold standard for the other three diagnostic strategies Blood spotted FTA cards were transferred from the field to the molecular laboratory at Makerere University College of Health Sciences in Kampala