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Fig. 1 | Malaria Journal

Fig. 1

From: High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories

Fig. 1

Establishment of the protocol. In a first step, tandem isolation of B cells and Th cells from whole blood was optimized and quality controlled for purity and efficiency by flow cytometry. Next, B cells and Th cells were isolated from small amounts of blood from healthy paediatric donors, cell numbers were determined and gene expression of various genes was analysed by qRT-PCR in order to determine the minimal amount of blood and cells necessary for reliable qRT-PCR results. Then, different preservation methods were compared under various conditions. Finally, the protocol was employed to analyse gene expression in B cells and Th cells from paediatric malaria patients isolated in a rural hospital in Uganda

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