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Fig. 3 | Malaria Journal

Fig. 3

From: High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories

Fig. 3

Cell number and Ct value analysis after B and Th cell isolation from whole blood of healthy paediatric donors. Blood was withdrawn from 13 healthy paediatric donors, B and Th cells were isolated and gene expression of AID, IL-4, TGF-b as well as the housekeeping gene HMBS was analysed by qRT-PCR. a B cell and Th cell numbers per ml of blood. b Amounts of blood withdrawn versus Ct values of HMBS (green), AID (red), IL-4 (orange) and TGF-b (blue). Symbol shapes indicate three different age groups. c Table summarizing parameters related to donors, B and Th cell isolation and gene expression analysis

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