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Fig. 4 | Malaria Journal

Fig. 4

From: High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories

Fig. 4

Evaluation of different preservation methods at various conditions. a, b A pellet of 10 × 106 cells or RNA of 10 × 106 cells, respectively, was stored as indicated (x-axis) for 1 month. a RIN were determined for every condition. Shown are individual data points, mean and SD of three experiments. b Ct values (y-axis) of three housekeeping genes and three B cell genes were measured by qRT-PCR for every condition. Shown are individual data points, mean and SD of three experiments. c Mean Ct values of three housekeeping genes and three B cell genes measured in samples stored in RNAstable (left panel) or RNAshield (right panel) at − 80 °C were correlated to the mean Ct values of the same genes stored at RT. d Upper left panel: schematic representation of sample storage and simulation of transient power disruption with corresponding estimated temperatures: samples were stored in RNAshield at either − 20 °C (black), 4 °C (red), 25 °C (blue) or 37 °C (green). At day 7, a power disruption was simulated for some of the samples (dotted lines) but not for other (solid lines), and at day 8, samples were placed back to their original storage temperature, except for sample c (dashed line). Lower left panel: RIN were determined for every condition. Shown are individual data points, mean and SD of three experiments. Right panel: Ct values (y-axis) of three housekeeping genes and three B cell genes were determined by qRT-PCR for every condition. Shown are individual data points, mean and SEM of three experiments

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