Assessment of drug resistance associated genetic diversity in Mauritanian isolates of Plasmodium vivax reveals limited polymorphism

Table 1 Primers used to amplify Pvdhfr, Pvdhps, Pvmdr1, and Pvk12

Gene	Primer sequences	Amplicon size (bp)^a
Pvdhfr
1st PCR	Forward: 5′-ACCCTTCCATAGGGAGTCCACTT-3′	961
1st PCR	Reverse: 5′-CGCATTGCAGTTCTCCGAA-3′
2nd PCR	Forward: 5′-CCCCACCACATAACGAAGTAG-3′	632
2nd PCR	Reverse: 5′-GCCGTTGATCCTCGTGAAG-3′
Pvdhps
1st PCR	Forward: 5′-GGAAGCCATTCGCTCAACTTATAA-3′	970
1st PCR	Reverse: 5′-CGTCAGTTTACCCTCCCCGTT-3′
2nd PCR	Forward: 5′-GATGGCGGTTTATTTGTCGAT-3′	767
2nd PCR	Reverse: 5′-GCCTCCCCGCTCATCAGTCT-3′
Pvmdr1
1st PCR	Forward: 5′-GCGAACTCGAATAAGTACTCCCTCTA-3′	762
1st PCR	Reverse: 5′-GGCGTAGCTTCCCGTAAATAAA-3′
2nd PCR	Forward: 5′-GGATTGCTGTCAGCACATATTAACA-3′	547
2nd PCR	Reverse: 5′AGAGGGATTTCATAAAGTCATCCACT-3′
Pvk12
1st PCR	Forward: 5′-ATCCAACAGCATTTCCAACT-3′	2108
1st PCR	Reverse: 5′-CAATTAAAACGGAATGTCCA-3′
2nd PCR	Forward: 5′-ACCACGTGACGAGGGATAAG-3′	1015
2nd PCR	Reverse: 5′-AAAACGGAATGTCCAAATCG-3′

^aPrimer sequences and amplicon sizes were from Mint Lekweiry et al. [3] for Pvdhfr, Pvdhps and Pvmdr1 and Popovici et al. [39] for Pvk12

ISSN: 1475-2875