Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

Priest, Jeffrey W.; Plucinski, Mateusz M.; Huber, Curtis S.; Rogier, Eric; Mao, Bunsoth; Gregory, Christopher J.; Candrinho, Baltazar; Colborn, James; Barnwell, John W.

doi:10.1186/s12936-018-2566-0

Table 4 Representative MSP1₁₉ competition assay results using sera from a high incidence setting

From: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP1₁₉ subunit proteins in multiplexed serologic assays

Sample	Competitor added	Pf MSP1₁₉ (MFI-bg)	Pm MSP1₁₉ (MFI-bg)	Po MSP1₁₉ (MFI-bg)	PvMSP1₁₉ (MFI-bg)	PfCSP (MFI-bg)	GST (MFI-bg)
Mozambique 6	PBS buffer only	28,163	93	75	83	24,603	22
	GST	28,217	79	57	56	24,181	21
	Pf MSP1₁₉	45 ^a	76	54	48	24,313	27
	Pm MSP1₁₉	28,133	16	54	42	24,467	25
	Po MSP1₁₉	28,237	71	32	47	24,533	21
	Pv MSP1₁₉	27,881	71	57	17	24,367	25
Mozambique 13	PBS buffer only	29,635	24,263	660	28,869	26,738	6
	GST	29,480	24,168	656	28,481	26,414	6
	Pf MSP1₁₉	38	24,276	594	28,799	26,792	6
	Pm MSP1₁₉	29,729	395	618	29,039	26,728	6
	Po MSP1₁₉	29,522	24,142	29	28,411	26,736	7
	Pv MSP1₁₉	29,724	24,182	637	122	26,670	7
Mozambique 17	PBS buffer only	27,423	1082	23,326	87	23,363	2
	GST	27,420	1164	23,551	100	23,351	5
	Pf MSP1₁₉	25	1189	23,599	104	23,481	3
	Pm MSP1₁₉	27,280	18	23,389	97	23,514	2
	Po MSP1₁₉	27,239	1157	16	94	23,468	4
	Pv MSP1₁₉	27,199	1173	23,387	29	23,329	4
Mozambique 1	PBS buffer only	29,855	5009	4945	27,283	22,826	10
	GST	29,797	5191	4942	27,173	22,987	9
	Pf MSP1₁₉	66	*3017* ^b	4998	26,901	22,818	11
	Pm MSP1₁₉	29,636	169	4841	26,949	22,820	8
	Po MSP1₁₉	29,597	4578	44	26,592	22,866	9
	Pv MSP1₁₉	29,693	4946	4703	57	22,879	8
Mozambique 20	PBS buffer only	26,789	27,032	491	1227	23,870	24
	GST	26,841	26,891	536	1384	24,149	11
	Pf MSP1₁₉	98	27,258	459	1301	24,083	30
	Pm MSP1₁₉	26,668	488 ^c	388	1084	23,921	20
	Po MSP1₁₉	26,842	27,236	29	1033	24,089	26
	Pv MSP1₁₉	26,785	27,183	352	108	23,745	22
Mozambique 16	PBS buffer only	27,518	6425	17,816	24,437	21,029	25
	GST	27,782	6588	18,263	24,430	21,019	17
	Pf MSP1₁₉	25	*2040*	18,892	24,275	21,320	20
	Pm MSP1₁₉	27,399	80	18,126	24,324	20,318	20
	Po MSP1₁₉	27,866	6361	113	21,301	21,114	23
	Pv MSP1₁₉	27,805	6029	*232*	2353	20,007	22
Mozambique 19	PBS buffer only	2677	2904	353	16,473	23,808	5
	GST	2977	3230	371	18,213	23,981	5
	Pf MSP1₁₉	12	3187	366	17,993	24,120	4
	Pm MSP1₁₉	2950	40	151 ^d	19,599	23,715	5
	Po MSP1₁₉	2931	2605	40	*8192*	24,276	5
	Pv MSP1₁₉	2648	2246	107	50	24,094	4

^aMultiplex bead assay responses that were completely suppressed by homologous protein competition are indicated in italics cells. These responses were below the respective cut-off values
^bMultiplex bead assay responses that, as a result of heterologous competition, decreased more than 30% relative to the PBS control but remained above the respective cut-off values are indicated in bold italics cells. These values indicate some level of cross-reactivity
^cMultiplex bead assay responses that were only partially reduced by homologous protein competition are indicated in underlined cells. These responses remained above the respective cut-off values and likely represent incomplete antibody blocking
^dMultiplex bead assay responses that were completely suppressed by heterologous protein competition are indicated in underlined italics cells. These responses were below the respective cut-off values

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