Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

Table 5 Mozambique blood spot elutions that demonstrate multiple cross-reacting MSP1₁₉ antibody responses

Sample	Competitor added	Pf MSP1₁₉ (MFI-bg)	Pm MSP1₁₉ (MFI-bg)	Po MSP1₁₉ (MFI-bg)	PvMSP1₁₉ (MFI-bg)	PfCSP (MFI-bg)	GST (MFI-bg)
Mozambique 3	PBS buffer only	24,960	13,703	11,965	21,795	23,643	4
	GST	19,926	*6961*	*6418*	*12,687*	23,442	2
	Pf MSP1₁₉	40 ^a	237 ^c	53	*232*	23,742	1
	Pm MSP1₁₉	*2211* ^b	17	40	*210*	23,283	2
	Po MSP1₁₉	*2487*	183	27	*227*	23,919	3
	Pv MSP1₁₉	*2506*	232	69	40	23,882	1
Mozambique 12	PBS buffer only	24,121	13,890	9743	10,178	25,839	20
	GST	22,343	*6172*	*4284*	*4944*	25,547	10
	Pf MSP1₁₉	62	62	36	175	25,874	10
	Pm MSP1₁₉	*1420*	22	31	156	25,062	6
	Po MSP1₁₉	*1488*	54	31	172	25,492	7
	Pv MSP1₁₉	*1726*	75	49	30	25,447	8

^aMultiplex bead assay responses that were completely suppressed by homologous protein competition are indicated in italics cells. These responses were below the respective cut-off values
^bMultiplex bead assay responses that, as a result of heterologous competition, decreased more than 30% relative to the PBS control but remained above the respective cut-off values are indicated in bold italics cells. These values indicate some level of cross-reactivity
^cMultiplex bead assay responses that were completely suppressed by heterologous protein competition are indicated in underlined cells. These responses were below the respective cut-off values

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