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Table 6 Affinity purification of MSP119 binding antibodies using magnetic bead capture

From: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

Sample

Description

Pf MSP119 (MFI-bg)

Pm MSP119 (MFI-bg)

Po MSP119 (MFI-bg)

Pv MSP119 (MFI-bg)

PfCSP (MFI-bg)

GST (MFI-bg)

Teta (MFI-bg)

Mozambique 13

No treatment

29,650

26,475

1445

28,371

26,857

12

2424

 

Post incubation with Pv coated beads

30,363

26,631

1612

15,734

28,008

13

3517

 

Antibody eluted from Pv coated beads

4

0

2

1061 b

0

1

2

Mozambique 16

No treatment

28,323

6713

18,227

24,238

18,909

28

24,114

 

Post incubation with Pv coated beads

29,956

7588

3001

15,417

22,871

34

26,239

 

Antibody eluted from Pv coated beads

0

3

229 c

2173

0

0

0

Mozambique 15

No treatment

28,351

1555

17,238

264

25,020

7

12,797

 

Post incubation with Pf coated beads

12,216

1928

20,876

328

28,102

7

16,773

 

Antibody eluted from Pf coated beads

4267

2

0

34

0

0

0

Mozambique 12

No treatment

25,821

15,187

10,551

11,465

27,920

38

25,409

 

Post incubation with Pf coated beads

3919

1213

806

1538

29,536

37

27,357

 

Antibody eluted from Pf coated beads

84

28

21

10

0

1

1

  1. aTetanus toxoid, a protein lacking GST, was used as an additional control
  2. bPositive antibody responses recognizing the same MSP119 antigen as that used in the capture assay are indicated in italics
  3. cPositive antibody responses recognizing an MSP119 antigen not used in the capture assay are indicated in bold italics