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Fig. 1 | Malaria Journal

Fig. 1

From: Levels of human proteins in plasma associated with acute paediatric malaria

Fig. 1

Overview of the antibody suspension bead array workflow and sample demographics. a Plasma samples were distributed into plates. b Samples were directly labelled with biotin. c 255 antibodies were diluted and distributed into plates. d Antibodies were coupled to magnetic, colour-coded beads, each with an individual bead ID. e 255 labelled samples were incubated with antibody-coupled beads. The beads were subsequently incubated together with a streptavidin-conjugated fluorophore. Two assay plates were used in this study since 541 samples were included. Detection was made using a flow cytometer-like instrument, registering signal intensity and bead ID by the utilization of two different lasers. f Information about sample groups and sex distribution within each sample group. g Age distributions were outlined for each sample group. h Different severe disease stratifications per sample within the severe malaria group illustrated as a line in the legend. A number (n = 49) of the samples exhibit more than one severe disease manifestation (see also Additional file 1)

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