Analysis | Primer and probe names | Sequences (5′–3′) | Length (bp) | GC (%) | Tm (°C) | Amplicon size (bp) |
---|---|---|---|---|---|---|
XL-PCR [29] | DPKup | 5′-GTTATCCCAGAAGGCTTTGCAGGCTTCA-3′ | 28 | 50.0 | 67.8 | 5100 |
DPKlow | 5′-GCCGACTGAGCCCTGGGAGGTAGGTA-3′ | 26 | 65.4 | 71.1 | ||
2D6dupl-F | 5′-CCTGGGAAGGCCCCATGGAAG-3′ | 21 | 66.7 | 65.5 | 3500 | |
2D6dupl-R | 5′-CAGTTACGGCAGTGGTCAGCT-3′ | 21 | 57.1 | 63.2 | ||
5′2D6*5 | 5′-CACCAGGCACCTGTACTCCTC-3′ | 21 | 61.9 | 62.7 | 3500 | |
3′2D6*5 | 5′-CAGGCATGAGCTAAGGCACCCAGAC-3′ | 25 | 60.0 | 67.9 | ||
Int2 | 5′2D6Int2 | 5′-TTTTGCACTGTGGGTCCTC-3′ | 19 | 52.6 | 58.5 | 1101 |
3′2D6Int2 | 5′-CAAGGTGGACACGGAGAAG-3′ | 19 | 57.9 | 58.4 | ||
Direct sequencing of PCR-reamplified products, DSP | 5′2D6Ex1 | 5′-GCACAGTCAACACAGCAGGT-3′ | 20 | 55.0 | 61.7 | 503 |
3′2D6Ex1 | 5′-AATGCCCTTCTCCAGGAAGT-3′ | 20 | 50.0 | 59.2 | ||
5′2D6Ex2 | 5′-TTCCTCCATCACAGAAGGTG-3′ | 20 | 50.0 | 57.4 | 501 | |
3′2D6Ex2 | 5′-CTCCCTAGTGCAGGTGGTTT-3′ | 20 | 55.0 | 59.9 | ||
5′2D6Ex34 | 5′-GTCTTCCCTGAGTGCAAAGG-3′ | 20 | 55.0 | 59.1 | 754 | |
3′2D6Ex34 | 5′-AGTGGGGTCTCCTGGAATG-3′ | 19 | 57.9 | 58.9 | ||
5′2D6Ex56 | 5′-GAGGGACTTGGTGAGGTCAG-3′ | 20 | 60.0 | 60.0 | 794 | |
3′2D6Ex56 | 5′-GACACTCCTTCTTGCCTCCT-3′ | 20 | 55.0 | 59.6 | ||
5′2D6Ex7 | 5′-ATGAACTTTGCTGGGACACC-3′ | 20 | 50.0 | 59.0 | 505 | |
3′2D6Ex7 | 5′-CCAGCCCTGCCTATACTCTG-3′ | 20 | 60.0 | 59.9 | ||
5′2D6Ex89 | 5′-TCTAGTGGGGAGACAAACCAG-3′ | 21 | 52.4 | 59.3 | 802 | |
3′2D6Ex89 | 5′-CTGAGGAGGATGATCCCAAC-3′ | 20 | 55.0 | 57.7 | ||
Allele-specific oligonucleotide probes real time SNPs genotyping, ASO | ||||||
 C100T | 5′2D6C100T | 5′-CCTGGTGGACCTGATGCA-3′ | 18 | 61.1 | 59.5 | 73 |
3′2D6C100T | 5′-CCCGGGCAGTGGCA-3′ | 14 | 78.6 | 58.7 | ||
2D6C100T_WT | 5′-CCTGGTGGGTAGCGTG-3′ | 16 | 69.0 | 51.1 | ||
2D6C100T_MT | 5′-CCTGGTGAGTAGCGTG-3′ | 16 | 63.0 | 48.5 | ||
 G1846A | 5′2D6G1846A | 5′-GACCCCTTACCCGCATCTC-3′ | 19 | 63.2 | 60.1 | 73 |
3′2D6G1846A | 5′-GCTCACGGCTTTGTCCAAGA-3′ | 20 | 55.0 | 61.5 | ||
2D6G1846A_WT | 5′-CCCCCAGGACGCC-3′ | 13 | 85.0 | 48.0 | ||
2D6G1846A_MT | 5′-CCCCCAAGACGCC-3′ | 13 | 77.0 | 46.0 | ||
 C2850T | 5′2D6C2850T | 5′-CCTGAGAGCAGCTTCAATGATGA-3′ | 23 | 47.8 | 61.3 | 67 |
3′2D6 C2850T | 5′-CCATCCCGGCAGAGAACAG-3′ | 19 | 63.2 | 60.7 | ||
2D6C2850T _WT | 5′-ACTATGCGCAGGTTC -3′ | 15 | 53.0 | 41.9 | ||
2D6C2850T _MT | 5′-CACTATGCACAGGTTC-3′ | 16 | 50.0 | 43.4 | ||
 G4180C | 5′2D6G4180C | 5′-CCACCATGGTGTCTTTGCTTTC-3′ | 22 | 50.0 | 60.9 | 67 |
3′2D6G4180C | 5′-GCACAGCACAAAGCTCATAGG-3′ | 21 | 52.4 | 60.4 | ||
2D6G4180C _WT | 5′-CTGGTGAGCCCATCC-3′ | 15 | 67.0 | 47.4 | ||
2D6G4180C _MT | 5′-CTGGTGACCCCATCC-3′ | 15 | 67.0 | 47.4 |