Table 2 Multiplex conditions for male–female assay
| Â | Concentration | Program | Â |
|---|---|---|---|
Female primers CCp4 | 900 nM | 55 °C 15 min | RT-step |
Female probe—Texas Red | 200 nM | 95 °C 1 min |  |
Male primers PfMGET | 225 nM | 95 °C 10 s | 45 cycles |
Male probe—FAM | 200 nM | 60 °C 1 min |  |
Input total nucleic acid | 5 µL |  |  |