Fig. 5

NO production by BMDM treated with HZ and cultured in medium enriched in l-Arg (a) or in HZ-conditioned medium (b). BMDM were plated overnight in either standard medium or medium enriched with l-Arg (l-Arg 10×). Respective media (standard medium or l-Arg 10×) were used for all the experimental steps described below. Cells were primed for 2 h, and then stimulated with medium, HZ (25 or 100 µM), MDP (2 µg/ml), or LPS (200 ng/ml) for 24 h. Levels of nitrite were determined, and fresh medium was replaced. After additional 24 h incubation (24 h + 24 h), the nitrite levels in the supernatants were determined again. ^*P < 0.05 and ^**P < 0.01 vs standard medium at each time point. Data displayed as averages ± SD of three independent experiments (a). BMDM were plated overnight in either standard medium (unconditioned medium, U-CM) or in HZ-conditioned medium (HZ-CM). Respective media (U-CM or HZ-CM) were used for all the experimental steps described below. Cells were primed for 2 h, and then stimulated with medium, HZ (25 or 100 µM), or MDP (1 or 2 µg/ml) for 24 h. Levels of nitrite were measured in supernatants. ^#P < 0.01 vs control medium, ^§P < 0.01 vs U-CM. Data displayed as averages ± SD of three independent experiments (b)