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Table 1 Outputs of the statistical models

From: Cryogenically preserved RBCs support gametocytogenesis of Plasmodium falciparum in vitro and gametogenesis in mosquitoes

Dependent variable → Gametocytemia in vitro (male + female stage V) Oocyst prevalence in mosquito midguts Sporozoite prevalence in salivary glands Oocyst abundance (infected and uninfected midguts) Oocyst intensity (infected midguts only)
Summary statistics → OR Std. error p-value OR Std. error p-value OR Std. error p-value IRR Std. error p-value IRR Std. error p-value
Fixed effects
 (Intercept) 0.00 0.00 < 0.001 1.09 0.52 0.86 0.82 0.50 0.75 1.96 0.85 0.43 5.19 0.48 < 0.001
 4 °C RBCs vs Cryo-RBCs 1.31 0.41 0.40 1.05 0.38 0.89 1.35 0.84 0.63 1.20 0.71 0.80 1.12 0.36 0.75
 Duration of storage 1.01 0.04 0.72 0.89 0.06 0.07 0.93 0.08 0.36 0.86 0.10 0.13 0.95 0.07 0.48
Experimental design
 Total flasks/mosquito infections (SMFAs), nested within experimental block 11 11 10a 11 11
 Total number of experimental blocks 5 5 5 5 5
 Total no. of RBC donors tested 4 4 4 4 4
 Total number of observations 51 283 235 283 157
Random effects
 Group structure and variance for random effects
  Flasks/SMFAs nested within experimental block 0.00 0.00 0.26 0.43 0.10
  Experimental block 0.16 0.35 0.00 0.77 0.40
  RBC donor 0.00 0.17 0.02 0.46 0.21
 Overdispersion tests
  Dispersion ratio 0.98 1.23 1.00 0.75 1.07
  p-value 0.50 0.29 0.40 1.00 0.27
 Notes
  GLMM family (link) Binomial (logit) Binomial (logit) Binomial (logit) Negative binomial (log) Negative binomial (log)
  1. IRR Incidence rate ratios, NA not applicable, OR odds ratios
  2. aNo of infections is lower as sporozoite prevalence was not available from 1 infection