Fig. 2

Confocal imaging of immunolocalized Saglin in the salivary glands from adult female Anopheles gambiae. a A low magnification image (×200) of medial and lateral lobes with evidence of Saglin only in the medial and proximal lateral lobes. b The same sample as in panel A but the fluorescence signal associated with Saglin localization in the medial gland was overexposed by adjusting the gain during signal processing. Only under these conditions could a weakly fluorescent signal be detected in the distal lateral lobe. c–f Selected 1 µm optical frontal sections through parts of the medial gland shown in a and b. c Shows digitally reconstructed sagital- and transverse-sections revealing the presence of Saglin only in basal regions. d–f Are successive sections beginning at the surface of the gland. The honeycomb appearance is due to the acinar structure of the gland, the presence of large secretory cavities and the basal distribution of Saglin. g, h Selected 1 µm optical frontal sections through parts of the proximal lateral gland where it joins with the distal lateral region shown in a and b. g shows digitally reconstructed sagittal- and transverse-sections revealing the presence of Saglin throughout the cytoplasm of the cells of this region. Red fluorescence (Alexafluor 555) indicates the presence of Saglin and blue fluorescence (DAPI) indicates DNA. Note that the salivary duct in the proximal lateral region non-specifically binds DAPI and does not indicate the presence of DNA