Fig. 1

Comparison of parasite outcomes based on observations with microscopy and the XN-30 analyzer. a (i) 24 h, (ii) 48 h. Upper and lower panels show microscopic images and M scattergrams obtained from the XN-30 analyzer, respectively. In the microscopic images, the scale bar represents 5 µm. In the scattergrams, the horizontal and vertical axes represent the intensities of side fluorescent light (SFL, which corresponds to DNA content) and forward scattered light (FSC, indicating size of iRBCs), respectively. The colours indicate the following: red, ring-form; orange, trophozoite; purple, schizont; and blue, polychromatic red blood cell (RBC). The colours were assigned based on the default setting of the XN-30 analyzer. b Parasitaemia measured with the XN-30 analyzer. Colours are as described above. ART artemisinin, AV atovaquone, CQ chloroquine, MQ mefloquine, PYR pyrimethamine. The original data obtained from the XN-30 analyzer are provided in Additional file 2: Table S1. All data were acquired using Plasmodium falciparum 3D7 with 5 µM of each anti-malarial drug