Fig. 4
Isothermal titration calorimetry studies of the cyclic (2035–2074) and linear PvRON2 peptides binding to PvAMA1-His6. Heat flow (upper panel) and integrated heat per injection (lower panel) along the titration of 9.43 µmol/L PvAMA1-His6 with the peptides (cyclic and linear) (10 µL injections of 300 µmol/L peptide). The heat of dilution was subtracted from the data. The line in the lower panel is a fit using the one-site model (ΔH = − 36 kcal/mol, K = 3 × 105 M−1, n = 0.6)