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Table 1 Comparison of kinetic data from Plasmodium vivax and Plasmodium falciparum

From: Glucose 6-phosphate dehydrogenase 6-phosphogluconolactonase: characterization of the Plasmodium vivax enzyme and inhibitor studies

 

PvG6PD

PfG6PD*

PfGluPho wt

PfGluPho wt*

hG6PD*

Vmax (U mg−1)

 G6P

5.6 ± 0.7

5.5 ± 0.2

5.9 ± 1.0

5.2 ± 1.6

64.4 ± 11

 NADP+

5.5 ± 0.4

5.5 ± 0.1

5.9 ± 0.6

4.6 ± 0.8

57.7 ± 15

KM (μM)

 G6P

80.2 ± 19.8

33.2 ± 1.2

20.3 ± 5.6

19.2 ± 3.9

116 ± 8.5

 NADP+

14.8 ± 2.9

6.1 ± 0.7

6.1 ± 1.3

6.5 ± 2.2

17.5 ± 2.8

kcat (s−1)

 G6P

6.7 ± 0.8

6.3 ± 0.3

10.7 ± 1.8

8.6 ± 1.5

64.6 ± 8.9

 NADP+

6.5 ± 0.4

6.3 ± 0.1

10.1 ± 0.9

8.2 ± 1.2

56.9 ± 15

Catalytic efficiency (L mol−1 s−1)

 G6P

8.2·104

1.9·105

5.3·105

4.4·105

5.6·105

  1. Values represent mean ± SD of least three independent determinations with different enzyme batches, each including at least two measurements
  2. * Values are taken from Jortzik et al. [14] for comparison. Due to the higher KM value for G6P in PvG6PD, 800 µM G6P was defined as substrate saturation. Catalytic efficiency (kcat/KM) was calculated from mean values to allow for comparison with the values published therein [14]