| PvG6PD | PfG6PD* | PfGluPho wt | PfGluPho wt* | hG6PD* |
---|---|---|---|---|---|
Vmax (U mg−1) | |||||
 G6P | 5.6 ± 0.7 | 5.5 ± 0.2 | 5.9 ± 1.0 | 5.2 ± 1.6 | 64.4 ± 11 |
 NADP+ | 5.5 ± 0.4 | 5.5 ± 0.1 | 5.9 ± 0.6 | 4.6 ± 0.8 | 57.7 ± 15 |
KM (μM) | |||||
 G6P | 80.2 ± 19.8 | 33.2 ± 1.2 | 20.3 ± 5.6 | 19.2 ± 3.9 | 116 ± 8.5 |
 NADP+ | 14.8 ± 2.9 | 6.1 ± 0.7 | 6.1 ± 1.3 | 6.5 ± 2.2 | 17.5 ± 2.8 |
kcat (s−1) | |||||
 G6P | 6.7 ± 0.8 | 6.3 ± 0.3 | 10.7 ± 1.8 | 8.6 ± 1.5 | 64.6 ± 8.9 |
 NADP+ | 6.5 ± 0.4 | 6.3 ± 0.1 | 10.1 ± 0.9 | 8.2 ± 1.2 | 56.9 ± 15 |
Catalytic efficiency (L mol−1 s−1) | |||||
 G6P | 8.2·104 | 1.9·105 | 5.3·105 | 4.4·105 | 5.6·105 |