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Table 1 Comparison of kinetic data from Plasmodium vivax and Plasmodium falciparum

From: Glucose 6-phosphate dehydrogenase 6-phosphogluconolactonase: characterization of the Plasmodium vivax enzyme and inhibitor studies

  PvG6PD PfG6PD* PfGluPho wt PfGluPho wt* hG6PD*
Vmax (U mg−1)
 G6P 5.6 ± 0.7 5.5 ± 0.2 5.9 ± 1.0 5.2 ± 1.6 64.4 ± 11
 NADP+ 5.5 ± 0.4 5.5 ± 0.1 5.9 ± 0.6 4.6 ± 0.8 57.7 ± 15
KM (μM)
 G6P 80.2 ± 19.8 33.2 ± 1.2 20.3 ± 5.6 19.2 ± 3.9 116 ± 8.5
 NADP+ 14.8 ± 2.9 6.1 ± 0.7 6.1 ± 1.3 6.5 ± 2.2 17.5 ± 2.8
kcat (s−1)
 G6P 6.7 ± 0.8 6.3 ± 0.3 10.7 ± 1.8 8.6 ± 1.5 64.6 ± 8.9
 NADP+ 6.5 ± 0.4 6.3 ± 0.1 10.1 ± 0.9 8.2 ± 1.2 56.9 ± 15
Catalytic efficiency (L mol−1 s−1)
 G6P 8.2·104 1.9·105 5.3·105 4.4·105 5.6·105
  1. Values represent mean ± SD of least three independent determinations with different enzyme batches, each including at least two measurements
  2. * Values are taken from Jortzik et al. [14] for comparison. Due to the higher KM value for G6P in PvG6PD, 800 µM G6P was defined as substrate saturation. Catalytic efficiency (kcat/KM) was calculated from mean values to allow for comparison with the values published therein [14]