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Table 2 Kinetic characteristics of P. falciparum GluPho variants

From: Glucose 6-phosphate dehydrogenase 6-phosphogluconolactonase: characterization of the Plasmodium vivax enzyme and inhibitor studies

  PfGluPho wt* PfGluPho wt PfGluPhoS315Y PfGluPhoL395F PfGluPhoF507L PfGluPhoS899E PfGluPhoS900E
Vmax (U mg−1)
 G6P 5.2 ± 1.6 5.9 ± 1.0 4.4 ± 1.1 4.5 ± 0.9 6.0 ± 1.4 5.6 ± 0.1 6.0 ± 1.1
 NADP+ 4.6 ± 0.8 5.9 ± 0.6 4.6 ± 1.5 4.0 ± 1.1 5.0 ± 0.3 5.2 ± 0.2 6.0 ± 0.6
KM (μM)
 G6P 19.2 ± 3.9 20.3 ± 5.6 23.4 ± 7.1 29.2 ± 4.1 24.1 ± 4.4 24.1 ± 2.8 18.4 ± 5.1
 NADP+ 6.5 ± 2.2 6.1 ± 1.3 5.1 ± 1.0 4.0 ± 0.2 3.7 ± 0.1 6.2 ± 0.5 5.8 ± 1.5
kcat (s−1)
 G6P 8.6 ± 1.5 10.7 ± 1.8 7.9 ± 2.0 8.1 ± 1.6 10.9 ± 2.5 10.1 ± 0.2 10.8 ± 1.9
 NADP+ 8.2 ± 1.2 10.1 ± 0.9 8.3 ± 2.7 7.2 ± 2.0 9.1 ± 0.5 9.3 ± 0.4 10.8 ± 1.2
Catalytic efficiency (L mol−1 s−1)
 G6P 4.4·105 5.8·105 ± 1.5∙105 3.7·105 ± 2.0∙105 2.9·105 ± 9.4∙104 4.5·105 ± 2.2∙104 5.2·105 ± 2.3∙105 6.2∙105 ± 2.2∙105
  1. Values represent mean ± SD of at least two independent determinations with different enzyme batches, each including at least two independent measurements
  2. * Values are from Jortzik et al. [14] for comparison. Catalytic efficiency (kcat/KM) from there [14] was calculated from mean values to allow for comparison