Table 2 Kinetic characteristics of P. falciparum GluPho variants
| Â | PfGluPho wt* | PfGluPho wt | PfGluPhoS315Y | PfGluPhoL395F | PfGluPhoF507L | PfGluPhoS899E | PfGluPhoS900E |
|---|---|---|---|---|---|---|---|
Vmax (U mg−1) | |||||||
 G6P | 5.2 ± 1.6 | 5.9 ± 1.0 | 4.4 ± 1.1 | 4.5 ± 0.9 | 6.0 ± 1.4 | 5.6 ± 0.1 | 6.0 ± 1.1 |
 NADP+ | 4.6 ± 0.8 | 5.9 ± 0.6 | 4.6 ± 1.5 | 4.0 ± 1.1 | 5.0 ± 0.3 | 5.2 ± 0.2 | 6.0 ± 0.6 |
KM (μM) | |||||||
 G6P | 19.2 ± 3.9 | 20.3 ± 5.6 | 23.4 ± 7.1 | 29.2 ± 4.1 | 24.1 ± 4.4 | 24.1 ± 2.8 | 18.4 ± 5.1 |
 NADP+ | 6.5 ± 2.2 | 6.1 ± 1.3 | 5.1 ± 1.0 | 4.0 ± 0.2 | 3.7 ± 0.1 | 6.2 ± 0.5 | 5.8 ± 1.5 |
kcat (s−1) | |||||||
 G6P | 8.6 ± 1.5 | 10.7 ± 1.8 | 7.9 ± 2.0 | 8.1 ± 1.6 | 10.9 ± 2.5 | 10.1 ± 0.2 | 10.8 ± 1.9 |
 NADP+ | 8.2 ± 1.2 | 10.1 ± 0.9 | 8.3 ± 2.7 | 7.2 ± 2.0 | 9.1 ± 0.5 | 9.3 ± 0.4 | 10.8 ± 1.2 |
Catalytic efficiency (L mol−1 s−1) | |||||||
 G6P | 4.4·105 | 5.8·105 ± 1.5∙105 | 3.7·105 ± 2.0∙105 | 2.9·105 ± 9.4∙104 | 4.5·105 ± 2.2∙104 | 5.2·105 ± 2.3∙105 | 6.2∙105 ± 2.2∙105 |