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Fig. 2 | Malaria Journal

Fig. 2

From: A fast and cost-effective microsampling protocol incorporating reduced animal usage for time-series transcriptomics in rodent malaria parasites

Fig. 2

Time-series transcriptome of Plasmodium vinckei vinckei CY. a Heat maps showing gene expression in P. vinckei at 6 h time points during the 24 h asexual cycle, each corresponding to a dominant population of rings (R), early trophozoites (E.T), late trophozoites (L.T) and schizonts (S) respectively. Gene expression values have been centered and normalized as Z-scores with the average level of expression among the genes set as zero (black) and higher and lower levels shown as red and green respectively. On the left panel, all significantly regulated P. vinckei genes (4328 genes) were ordered from top to bottom according to their phase of expression to create a phaseogram. On the right panel, P. vinckei genes with one-to-one orthologs in P. falciparum 3D7 (2480 genes) were ordered based on P. falciparum 3D7 phaseogram shown in [1]. Both kinds of ordering display the transcriptional cascade in P. vinckei, closely reflecting that of P. falciparum. Gene-wise FPKM values are given in Additional files 3 and 4). b Expression profiles of RNA polymerases (known to peak during ring and early trophozoite stages) and invasion-related genes (known to peak during late trophozoite and schizont stages) in P. vinckei vinckei CY. Blue and orange lines represent genes clustered with different expression profiles and the black line represents average trend of expression in a particular gene cluster (fpkm—fragments per kilobase of transcript per million mapped reads). Gene lists are given in Additional file 5

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