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Table 1 Microsample characteristics and RNA-seq mapping statistics

From: A fast and cost-effective microsampling protocol incorporating reduced animal usage for time-series transcriptomics in rodent malaria parasites

RMP Microsample Parasitaemia RNA yield (μg) Total reads Parasite reads Mapping percentage Median fragment coverage Median TIN
P. chabaudi Sample I 7.74 0.87 26,585,563 1,228,998 4.62 8 87.66
Sample II 6.22 0.42 19,842,987 2,458,755 12.39 49 86.41
Sample III 5.75 1.71 15,332,092 676,488 4.41 10 92.2
Sample IV 3.64 1.03 31,385,250 1,420,373 4.52 26 91.13
P. vinckei 6h_rep1 23.88 9.6 32,755,140 22,915,171 69.96 499 91.27
6h_rep2 22.19 9 31,427,694 21,274,632 67.69 479 91.15
6h_rep3 23.14 9.03 31,331,956 20,044,950 63.98 424 91.04
12h_rep1 24.19 11.49 35,255,928 26,290,657 74.57 710.5 91.79
12h_rep2 24.32 11.46 28,504,674 16,567,841 58.12 451.5 86.61
12h_rep3 22.75 10.14 27,368,020 17,521,494 64.02 483.5 92
18h_rep1 24.77 6.33 27,622,130 18,733,720 67.82 582 89.88
18h_rep2 25.65 11.49 29,253,240 18,590,556 63.55 552 91.11
18h_rep3 23.11 10.23 27,377,938 15,234,884 55.65 468 90.08
24h_rep1 27.06 5.4 33,624,482 25,522,094 75.90 698 91.57
24h_rep2 25.92 1.24 35,255,928 20,282,255 57.53 501.5 87.43
24h_rep3 26.45 1.95 35,255,928 23,045,897 65.37 634 90.69
  1. Microsamples from Plasmodium chabaudi AS had low parasitaemia and therefore, a low percentage of reads mapping to the P. chabaudi genome. In contrast, Plasmodium vinckei vinckei CY microsamples had lesser host contamination resulting in higher median fragment coverage across its transcripts. Transcript integrity number (TIN) was calculated using RSeQC [51] and all samples showed a high TIN value, indicating little to no evidence of RNA degradation