Fig. 3From: In vivo compartmental kinetics of Plasmodium falciparum histidine-rich protein II in the blood of humans and in BALB/c mice infected with a transgenic Plasmodium berghei parasite expressing histidine-rich protein IICharacterization of the synthesis and localization of PfHRP2 by transgenic PbPfHRP2 parasite. a BinaxNOW Malaria RDT™ detecting tgPfHRP2 and Plasmodium aldolase in the whole blood of a PbPfHRP2 infected mouse. b tgPfHRP2 was detected in iRBCs, and exported to the iRBC cytoplasm (Saponin, SN). RBCs were lysed with saponin to isolate their cytoplasmic fraction. Purified recombinant and native PfHRP2 protein were run as controls. c tgPfHRP2 (red) localized within the parasite, demarcated by Plasmodium enolase staining (green), and the RBC cytosol, similar to what was observed in P. falciparum iRBCs. An uninfected mouse RBC and a P. berghei ANKA iRBC that does not produce PfHRP2 are shown as controlsBack to article page