Fig. 4

Kinetics of tgPfHRP2 following PbPfHRP2 infection. Tail blood was extracted daily from PbPfHRP2 infected female BALB/c mice post-treatment with artesunate and pyronaridine administered for cure (n = 11). Blood was fractionated into plasma and RBCs. a Parasite genomic DNA clearance post-treatment was quantified using qPCR, with TaqMan primers and probes specific for P. berghei ANKA 18S rRNA. Data are represented as mean ± SEM. b tgPfHRP2 protein levels post-treatment were quantified in the two blood fractions by ELISA. Data are represented as mean ± SEM. c The ratio of tgPfHRP2 concentration in the RBCs to plasma in each mouse was calculated over the first 4 days post-treatment. Data are represented as mean ± SEM. d The half-life of the tgPfHRP2 was quantified in the plasma and washed RBCs using a first-order decay equation model constrained at a plateau of Y = 0. Each point represents an individual mouse with mean ± SEM displayed. Data were analysed using a Wilcoxon signed-rank test