Fig. 8

Consequences of hypoxanthine deprivation on parasite metabolism, as captured in model simulations. These are most clearly delineated in model simulations of parasite metabolism during the first 24 h of hypoxanthine deprivation. The simulations indicated that the parasite supplies phosphoenolpyruvate (PEP) to synthesize chorismate, which is a precursor for ubiquinone synthesis as part of shikimate synthesis. PEP is also utilized to synthesize oxaloacetate (OAA), which in turn leads to enhanced porphyrin metabolism. The red arrows indicate the metabolic flow occurring under hypoxanthine-deprivation conditions. A dotted arrow indicates more than one reaction step. AKG, alpha-ketoglutarate; ETC, electron transport chain; FUM, fumarate; MAL, l-malate; nad, oxidized nicotinamide adenine dinucleotide; nadp, oxidized nicotinamide adenine dinucleotide phosphate; nadph, reduced nadp; PPP, pentose phosphate pathway; SUC, succinate; q8, ubiquinone