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Fig. 1 | Malaria Journal

Fig. 1

From: Sensitive real-time PCR detection of Plasmodium falciparum parasites in whole blood by erythrocyte membrane protein 1 gene amplification

Fig. 1

Abundance of PfEMP1-specific amplicon in Plasmodium falciparum genome. Primers used in the RT-PCR assay amplify a region of 260 nucleotides located in the cytosolic acidic terminal segment (ATS) region of PfEMP1. Matching conserved sequences in the P. falciparum (3D7) genome were identified using an NCBI BLAST search. Chromosomes with homologous sequences are displayed in the figure. The conserved amplification region was found in 18 different loci reflecting significantly higher abundance than the 5 copies of the 18S gene in the P. falciparum genome. Black bars represent forward and reverse primer binding positions

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Malaria Journal

ISSN: 1475-2875

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