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Fig. 3 | Malaria Journal

Fig. 3

From: Sensitive real-time PCR detection of Plasmodium falciparum parasites in whole blood by erythrocyte membrane protein 1 gene amplification

Fig. 3

Determination of analytical sensitivity for Plasmodium falciparum-infected red blood cells by probit analysis. gDNA extracted from 30 independent 1 mL aliquots taken from the stock parasite dilutions and analysed by RT-PCR using both primer sets. Probit regression was performed using the MedCalc statistical software to identify the 95% reactive threshold. a The 18S primer set demonstrated a 95% reactivity of 98.2 parasites/mL (95% CI 64.5–131.9). b PfEMP1 primer set demonstrated a 95% threshold of 9.3 parasites/mL (95% CI 6.36–17.8)

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Malaria Journal

ISSN: 1475-2875

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