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Fig. 4 | Malaria Journal

Fig. 4

From: Sensitive real-time PCR detection of Plasmodium falciparum parasites in whole blood by erythrocyte membrane protein 1 gene amplification

Fig. 4

Detection of Plasmodium falciparum in dried blood spots from blood smear positive individuals in Ghana. a The 18S primer set detected only the highest parasite dilution (41,360 parasites/mL) while the PfEMP1 primer set detected the lowest parasite concentration tested corresponding to 2640 parasites/mL. b The 18S primer set identified 55% (11/20) smear positive samples as positive whereas the EMP1 primer set identified 100% (20/20) of smear positive samples as positive. gDNA was extracted from sera demonstrating high reactivity (OD450 ≥ 1) against P. falciparum circumsporozoite protein measured in ELISA and amplified using both primer sets, c Pf18S primers failed to amplify from any of the sera samples. d PfEMP1 primer set identified 5/7 (71.4%) sera samples as positive for P. falciparum nucleic acid

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Malaria Journal

ISSN: 1475-2875

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