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Fig. 5 | Malaria Journal

Fig. 5

From: Sensitive real-time PCR detection of Plasmodium falciparum parasites in whole blood by erythrocyte membrane protein 1 gene amplification

Fig. 5

Detection of Plasmodium falciparum in asymptomatic blood smear negative individuals from Ghana. gDNA was extracted from whole blood samples collected from children and adults who exhibited no symptoms of malaria and had negative blood smears. Pf18S primers identified 53/98 (54.1%) of samples as positive for P. falciparum gDNA whereas PfEMP1 primers identified 69/98 (70.4%) blood samples as positive. These differences are statistically significant when evaluated by McNemar’s test and demonstrate PfEMP1 primers possess superior sensitivity for the detection of low burden parasitaemia and more comprehensive identification of infectious reservoir

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Malaria Journal

ISSN: 1475-2875

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