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Table 1 Prevalence and performance of diagnostic tests for detection of Plasmodium infection in school-aged children

From: Non-invasive surveillance of Plasmodium infection by real-time PCR analysis of ethanol preserved faeces from Ugandan school children with intestinal schistosomiasis

School name

Walukuba (n = 43)

Runga (n = 50)

Bugoigo (n = 56)

Biiso (n = 49)

Busingiro (n = 49)

Total (n = 247)

n (%)

n (%)

n (%)

n (%)

n (%)

n (%)

Prevalence

 Rapid diagnostic testa

  Negative

29 (67.4)

21 (42.0)

26 (46.4)

16 (32.6)

17 (34.6)

109 (44.1)

  Positive

14 (32.5)

29 (58.0)

30 (53.5)

33 (67.3)

32 (65.3)

138 (55.8)

  95% CI

[20.4–47.4]

[44.2–70.6]

[40.7–65.9]

[53.3–78.7]

[51.3–77.0]

[49.6–61.9]

 rtPCR-based assays for DBSb

  Negative

10 (23.2)

8 (16.0)

24 (42.8)

5 (10.2)

2 (4.0)

49 (19.8)

  Positive

33 (76.7)

42 (84.0)

32 (57.1)

44 (89.8)

47 (95.9)

198 (80.1)

  95% CI

[62.2–86.8]

[71.4–91.6]

[44.1–69.2]

[78.2–95.5]

[86.2–98.8]

[74.7–84.6]

 rtPCR-based assays for EPFc

  Negative

28 (65.1)

31 (62.0)

13 (23.2)

4 (8.1)

13 (26.5)

89 (36.0)

  Positive

15 (34.8)

19 (38.0)

43 (76.7)

45 (91.8)

36 (73.4)

158 (63.9)

  95% CI

[22.4–49.8]

[25.8–51.8]

[64.2–85.9]

[80.8–96.7]

[59.7–83.7]

[57.8–69.7]

No. of infected

(n = 33)

(n = 42)

(n = 32)

(n = 44)

(n = 47)

Total (n = 198)

 Co-infection (SCH, MALd)

  Negative

4 (12.1)

6 (14.2)

21 (65.6)

36 (81.8)

43 (91.4)

110 (55.5)

  Positive

29 (87.8)

36 (85.7)

11 (34.3)

8 (18.1)

4 (8.5)

88 (44.5)

  95% CI

[72.6–95.1]

[72.1–93.2]

[20.4–51.6]

[9.5–31.9]

[3.3–19.9]

[37.6–51.4]

  1. aMalaria by RDT (SD Bioline Malaria Ag P.f/Pan test, SD Diagnostics, Yongin-si, Gyeonggi-do, Korea)
  2. bA TaqMan-based real-time PCR assay that collectively detected all 4 Plasmodium species from DBS
  3. cA TaqMan-based real-time PCR assay that collectively detected all 4 Plasmodium species from EPF
  4. dCo-infections among schoolchildren with Plasmodium species detected by rtPCR-based assay for DBS and Schistosoma mansoni by Kato-Katz faecal microscopy