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Table 1 Prevalence and performance of diagnostic tests for detection of Plasmodium infection in school-aged children

From: Non-invasive surveillance of Plasmodium infection by real-time PCR analysis of ethanol preserved faeces from Ugandan school children with intestinal schistosomiasis

School name Walukuba (n = 43) Runga (n = 50) Bugoigo (n = 56) Biiso (n = 49) Busingiro (n = 49) Total (n = 247)
n (%) n (%) n (%) n (%) n (%) n (%)
Prevalence
 Rapid diagnostic testa
  Negative 29 (67.4) 21 (42.0) 26 (46.4) 16 (32.6) 17 (34.6) 109 (44.1)
  Positive 14 (32.5) 29 (58.0) 30 (53.5) 33 (67.3) 32 (65.3) 138 (55.8)
  95% CI [20.4–47.4] [44.2–70.6] [40.7–65.9] [53.3–78.7] [51.3–77.0] [49.6–61.9]
 rtPCR-based assays for DBSb
  Negative 10 (23.2) 8 (16.0) 24 (42.8) 5 (10.2) 2 (4.0) 49 (19.8)
  Positive 33 (76.7) 42 (84.0) 32 (57.1) 44 (89.8) 47 (95.9) 198 (80.1)
  95% CI [62.2–86.8] [71.4–91.6] [44.1–69.2] [78.2–95.5] [86.2–98.8] [74.7–84.6]
 rtPCR-based assays for EPFc
  Negative 28 (65.1) 31 (62.0) 13 (23.2) 4 (8.1) 13 (26.5) 89 (36.0)
  Positive 15 (34.8) 19 (38.0) 43 (76.7) 45 (91.8) 36 (73.4) 158 (63.9)
  95% CI [22.4–49.8] [25.8–51.8] [64.2–85.9] [80.8–96.7] [59.7–83.7] [57.8–69.7]
No. of infected (n = 33) (n = 42) (n = 32) (n = 44) (n = 47) Total (n = 198)
 Co-infection (SCH, MALd)
  Negative 4 (12.1) 6 (14.2) 21 (65.6) 36 (81.8) 43 (91.4) 110 (55.5)
  Positive 29 (87.8) 36 (85.7) 11 (34.3) 8 (18.1) 4 (8.5) 88 (44.5)
  95% CI [72.6–95.1] [72.1–93.2] [20.4–51.6] [9.5–31.9] [3.3–19.9] [37.6–51.4]
  1. aMalaria by RDT (SD Bioline Malaria Ag P.f/Pan test, SD Diagnostics, Yongin-si, Gyeonggi-do, Korea)
  2. bA TaqMan-based real-time PCR assay that collectively detected all 4 Plasmodium species from DBS
  3. cA TaqMan-based real-time PCR assay that collectively detected all 4 Plasmodium species from EPF
  4. dCo-infections among schoolchildren with Plasmodium species detected by rtPCR-based assay for DBS and Schistosoma mansoni by Kato-Katz faecal microscopy