Table 1 Primers for species identification used for PET-PCR
Primer name | Sequence (5′–3′) |
|---|---|
Original genus 18sFor | GGC CTA ACA TGG CTA TGA CG |
Original genus FAM 18sRev | FAM-aggcgcatagcgcctggCTGCCTTCCT TAG ATGTGG TAG CT |
Falciparum For | ACC CCTCGCCTG GTG TTT TT |
Falciparum Rev | HEX-aggcggataccgcctggTCGG GCC CCA AAA ATA GGA A |
P. vivax For | GTA GCC TAAGAAGGC CGT GT |
P. vivax Rev | HEX-aggcgcatagcgcctggCCTGGGG GAT GAA TAT CTC TAC AGC ACT GT |
P. malariae For | AAGGCAGTAACACCAGCAGTA |
P. malariae Rev (based on dihydrofolate reductase-thymidylate synthase (DHFR-TS) gene) | FAM-aggcgcatagcgcctggTCCCATGAAGTTATATTCCCGCTC |
P. ovale For | FAM-aggcgcatagcgcctggCCACAGATAAGAAGTCTCAAGTACGATATT |
P. ovale Rev | TTGGAGCACTTTTGTTTGCAA |