Table 1 Primers used for qPCR verification for the potential genes associated with pyrethroid resistance, detected by RNA-seq
Genes | Primer names | Primer sequence (5′–3′) | Amplification length (bp) |
|---|---|---|---|
AsHSP90AB | HSP90AB-QF | GAAGATGGACATTATGGACGCAG | 161 |
HSP90AB-QR | GCGAGATGTCGTCGGCATTG | ||
AsHSP90AA | HSP90AA-QF | CAAGGATGATGAGCCGAAGC | 148 |
HSP90AA-QRÂ | CAATGCCGACGACATCTCGC | ||
AsHSP90B1 | HSP90B1-QF | GAGGGCGAGGTTACGTTCAA | 153 |
HSP90B1-QR | ACTCGTCCGTGATGAACACC | ||
AsTRAP | TRAP-QF | CGAATCCGGGAGGTAATCCG | 121 |
TRAP-QR | CCCACGAACCGGTAGAACTC | ||
AsHSP70-2 | HSP70-2-QF | CCCTCTTTTCTGGCTTCGGA | 127 |
HSP70-2-QR | AATGGTAGCGTGAGGAAGCC | ||
AsDNAJB4 | DNAJB4-QF | TCACACCTCTGCATTGGGTC | 136 |
DNAJB4-QR | ATCCCCACGAGGAAAGGCTA | ||
AsHSP21.7 | HSP21.7-QF | CGAATCCGGGAGGTAATCCG | 151 |
HSP21.7-QR | CCCACGAACCGGTAGAACTC | ||
AsHSP13.7 | HSP13.7-QF | TTCGGACGGTATCCTGACCA | 117 |
HSP13.7-QR | GCAGACTGTCCGTTCTCCAG | ||
AsHSP17.8 | HSP17.8-QF | GGACGAGCATGGCTACATCT | 106 |
HSP17.8-QR | TCCTCTCTGGGGCAAGTGAT | ||
AsHSP21 | HSP21-QF | ATGAACCGCGAGTCCAACTT | 128 |
HSP21-QR | TGGCGAGCTAATATCGGACG | ||
AsHSP23.5 | HSP23.5-QF | ATGTACTTCCGCGACTGGTG | 148 |
HSP23.5-QR | TACCGAAATCACTCCACGGC | ||
AsHSP24.5 | HSP24.5-QF | CAAAATCAGCATCCGGGTCG | 125 |
HSP24.5-QR | GTGTCGCGAGATGTAGCCAT | ||
AsHSP24.7 | HSP24.7-QF | CAGGACGAGCATGGCTACAT | 151 |
HSP24.7-QR | TCACAGCTTCTTTGGCAGAC | ||
AsHSP25.6 | HSP25.6-QF | TCGGACGGTATCCTGACCAT | 137 |
HSP25.6-QR | TCCATTTTCTCTCCTTCGGGC | ||
AsHSP19 | HSP19-QF | TGTTGATGAGCTCTGTGCTG | 122 |
HSP19-QR | TGGCGAGCTAATATCGGACG | ||
AsRPL49 | RPL49-QF | GGAGCCGGTCGGTGATATGT | 121 |
RPL49-QR | TTCCTTCTCGGTCGGCTTCG | ||
AsRPS7 | RPS7-QF | CGGAGAAGATGGCATGGGAGAT | 148 |
RPS7-QR | ATAGTGAGCATAGGCCCGGTTA |