Fig. 2
From: Hospital-derived antibody profiles of malaria patients in Southwest India
Protein microarray slide map. a Schematic representation of experiment design and workflow. b Scanned microarray pad probed with malaria positive patient sera (i) and no sera control probed with buffer alone (ii). A total of 500 P. falciparum and 515 P. vivax proteins were expressed as polypeptide fragments and printed as cell-free in vitro transcription translation (IVTT) reactions in four blocks. The pad also contains control spots; 24 IgG mix in six different concentrations (6 spots in each block), 24 anti-human IgG spots in six different concentrations, 32 TTBS (buffer) spots, 24 “No DNA-control” reaction spots lacking a DNA template in the plasmid vector, 7 blank and 6 empty spots arranged in four blocks (highlighted). 12 purified parasite proteins are printed in duplicates at two concentrations to serve as controls (printed between ‘No DNA’ control and TTBS spots)