Fig. 3

Seroreactivity and breadth of antibody response to P. falciparum and P. vivax malaria. a Heat map showing median normalized signal intensity values (MNSI) of 96 P. falciparum and 100 P. vivax patients to 500 P. falciparum and 515 P. vivax polypeptides, respectively. The three-colour gradient indicates high, intermediate and low median normalized signal intensity values. Red indicates highest values above 40,000, while white indicates no reactivity. Patients (columns) are ranked from left to right based on total mean values of antibody binding to all printed polypeptides. Polypeptides are ranked from top to bottom based on their mean values of antibody binding for all serum samples (Samples and polypeptides segregated according to parasite species). *(red)- IgG, anti-human IgG and postive controls, *(green)- 24 No DNA-controls. b Bar graph indicates the total number of seroreactive antigens in both study groups (278 P. falciparum and 73 P. vivax). The colour scale indicates the number of patients (range) seroreactive to a particular percentage of P. falciparum and P. vivax antigens. (*only in P. falciparum, ^#only in P. vivax). c Box plots describe antibody breadth to 500 P. falciparum and 515 P. vivax antigens. The box represents the interquartile range (IQR) with the horizontal line as the median value. The whiskers represent the minimum and maximum values from the data