Fig. 1

Anti-rPfCelTOS IgG antibody levels in vaccine mouse sera at different time points after immunization by ELISA. Anti-rPfCelTOS IgG antibody levels were compared between the collected sera from immunized mice on days 10, 24 (10 days after the first boost), and 38 (10 days after the second boost) of the first immunization. The sera samples were incubated in duplicate wells with 1:200 diluted sera for 90 min. There was a significant difference in total IgG antibody levels between different immunization time points in vaccine groups (paired sample t-test, P < 0.0001). The highest level of anti-rPfCelTOS IgG antibody was identified in the groups that received rPfCelTOS in combination with CpG + Poly I:C, (P < 0.0001, one-way ANOVA). Data represents means from three separate ELISA experiments. The bars and error bars show the mean OD_490nm and SD of 16 individual mice in each group, respectively. The ELISA cut-offs were calculated as the mean OD_490nm of NMS (as the negative controls, n = 20) plus 3 SD. The cut-offs for total IgG on days 10, 24, and 38 were almost 0.109, 0.099 and 0.101, respectively. ***P < 0.0001. Ag rPfCelTOS antigen, NMS normal mouse sera