| Immunization formulation | Antigen/adjuvant(s) |
---|
rPfCelTOS (µg/mouse) | CpG (µg/mouse) | Poly I:C (µg/mouse) | CFA/IFA (µL) |
---|
Prime | Boosts |
---|
Vaccine groups
|
1 | rPfCelTOS (Ag) | 10 | 5 | – | – | – |
2 | Ag/CpG | 10 | 5 | 10 | – | – |
3 | Ag/Poly I:C | 10 | 5 | – | 10 | – |
4 | Ag/CpG + Poly I:C | 10 | 5 | 5 | 5 | – |
5 | Ag/CFA/IFA | 10 | 5 | – | – | 100 |
Control groups
|
6 | CpG | – | – | 10 | – | – |
7 | Poly I:C | – | – | – | 10 | – |
8 | CpG + Poly I:C | – | – | 5 | 5 | – |
9 | CFA/IFA | – | – | – | – | 100 |
10 | PBS 1× | – | – | – | – | – |
- Mice received 200 μL rPfCelTOS (10 μg at prime and 5 μg at boost) alone (non-adjuvanted vaccine group) and in the presence of CpG (10 μg/mouse, vaccine grade type, InvivoGen, San Diego, CA, USA), Poly I:C (10 μg/mouse, vaccine grade type, InvivoGen, San Diego, CA, USA), and combination of both CpG (5 μg/mouse) and Poly I:C (5 μg/mouse) adjuvants (adjuvanted vaccine groups) subcutaneously. Also, the mice were immunized with rPfCelTOS emulsified in CFA (complete Freund’s adjuvant) as reference adjuvant (the first dose) or IFA (incomplete Freund’s adjuvant; subsequent doses), with 1:1 volume/volume ratio. Control mice immunized with sterile PBS 1× (pH 7.4), CFA/IFA (as the reference adjuvant), CpG, Poly I:C, and CpG + Poly I:C. Sera samples were collected from the tail vein 10 days after each immunization (days 10, 24, and 38 after the first immunization)
- Ag rPfCelTOS antigen, CFA complete Freund’s adjuvant, IFA incomplete Freund’s adjuvant, CpG synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs, Poly I:C polyinosinic:polycytidylic acid