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Fig. 1 | Malaria Journal

Fig. 1

From: Ribosomal and non-ribosomal PCR targets for the detection of low-density and mixed malaria infections

Fig. 1

Methodological strategy for field evaluation of PCR-based protocols. Regardless the results obtained by light microscopy, blood-derived DNA samples from clinical (n = 110) and subclinical (n = 324) malaria suspects were submitted to species-specific PCR based-protocols targeting ribosomal (18S rRNA gene) and non-ribosomal Plasmodium sequences. The 18S rRNA-based protocols included a Nested-PCR assay adapted from the original protocol (16) with modifications (22), and a real-time PCR assay (R-qPCR) as previously described (17). The non-ribosomal (NR) amplification of P. vivax (Pvr47) and P. falciparum (Pfr364) involved a previously described single step conventional PCR assay (NR-cPCR) (24), and a real-time PCR protocol (NR-qPCR) whose primers and cycling conditions were described in “Methods”

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