Plasmodium knowlesi clinical isolates from Malaysia show extensive diversity and strong differential selection pressure at the merozoite surface protein 7D (MSP7D)

Table 1 Estimates of nucleotide diversity, natural selection, haplotype diversity and neutrality indices of pkmsp7

Region	No. samples	SNPs	No. haplotype	Diversity ± SD		d_N − d_S	Codon-based z test	Taj D	Fu and Li’s D*	Fu and Li’s F*
Region	No. samples	SNPs	No. haplotype	Haplotype	Nucleotide	d_N − d_S	Codon-based z test	Taj D	Fu and Li’s D*	Fu and Li’s F*
Full-length	36	203	34	0.998 ± 0.007	0.052 ± 0.002	− 2.0	P > 0.1	0.295	0.190	0.270
5′ region		28	30	0.992 ± 0.009	0.010 ± 0.001	− 3.0	P > 0.1	− 1.25	− 2.24	− 2.26
Central region		151	32	0.995 ± 0.008	0.1495 ± 0.006	1.84	P < 0.05	0.673	0.896	0.973
3′ region		32	30	0.990 ± 0.010	0.017 ± 0.0007	− 2.68	P < 0.01	− 0.275	− 1.027	− 0.917

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