Evidence of asymptomatic submicroscopic malaria in low transmission areas in Belaga district, Kapit division, Sarawak, Malaysia

Table 1 List of primers including name, nucleotide sequence, PCR step, fragment size and specificity of the nested PCR [6, 8, 12]

	Plasmodium spp.	Size (bp)	Primer name	Primer sequences	PCR (T)
Nested 1st round	Genus-specific	1600–1700	rPLU1 rPLU5	TCAAAGATTAAGCCATGCAAGTGA CCTGTTGTTGCCTTAAACTTC	56 °C
Nested 2nd round	Genus-specific	235	rPLU3 rPLU4	TTTTTATAAGGATAACTACGGAAAAGCTGT CCCGTCATAGCCATGTTAGGCCAATACC	59 °C
Nested 2nd round species specific	P. vivax	121	rVIV1 rVIV2	CGCTTCTAGCTTAATCCACATAACTGATAC ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA	60 °C
	P. falciparum	206	rFAL1 rFAL2	TTAAACTGGTTTGGGAAAACCAAATATATT ACACAATGAACTCAATCATGACTACCCGTC
	P. ovale	226	rOVA1 rPLU2	ATCTCTTTTGCTATTTTTTAGTATTGGAGA ATCTAAGAATTTCACCTCTGACATCTG
	P. malariae	145	rMAL1 rMAL2	ATAACATAGTTGTACGTTAAGAATAACCCC AAAATTCCCATGCATAAAAATTATACAAA
	P. knowlesi	153	Pmk8 Pmkr9	GTTAGCGAGAGCCACAAAAAAGCGAAT ACTCAAAGTAACAAAATCTTCCGTA	61 °C

ISSN: 1475-2875