Fig. 4

Structural-based multiple sequence alignment of FP-2, FP-3 and homologs prodomain-catalytic domains. Actual residue numbering per protein is given on the side, and the top numbering is based on partial zymogen alignment. The papain family characteristic prodomain ERFNIN and GNFD motif residues are indicated with an asterisk. Bold short lines depict the prodomain-catalytic domain border. Dashed green lines indicate the position of α-helix and arrows β-sheet structural elements. Fully conserved residues in all the proteins are marked with red while residues only conserved in plasmodial proteases with blue. Position of subsite residues is shown with filled circles (Red = S1, Blue = S2, Green = S3 and S1′ = black)