Fig. 2

The figure shows the nested species-specific PCR for P. ovale. Lanes 1–4 correspond to the P. vivax reaction, lanes 5–8 correspond to the P. falciparum reaction and lanes 9–12 correspond to the P. ovale specific reaction. P. ovale was detected on MIS2595, whereas MIS2712 was only positive for Plasmodium spp. (≈ 1200 bp). We later determined via sequencing that MIS2712 was P. ovale wallikeri which is not detected by the PCR method we employed here