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Fig. 2 | Malaria Journal

Fig. 2

From: An improved nucleic acid extraction method from dried blood spots for amplification of Plasmodium falciparum kelch13 for detection of artemisinin resistance

Fig. 2

The current method outperforms Qiagen based kits for amplification of k13 from dried blood spots. a The optimized protocol (compatible with Omega EZ and Nunc 96-well DNA plates) is able to amplify k13 from all 5000 parasites/mL DBS samples, whereas Qiagen QIAamp and Investigator kits cannot. b Nunc DNA plates show improved sensitivity for amplification of 1000 parasites/mL DBS samples than Omega EZ DNA plates. M marker, GuSCN guanidine thiocyanate, ISOH isopropanol

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Malaria Journal

ISSN: 1475-2875

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