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Table 2 Addition of 16.7% isopropanol to the lysis buffer improves recovery of parasite DNA

From: An improved nucleic acid extraction method from dried blood spots for amplification of Plasmodium falciparum kelch13 for detection of artemisinin resistance

  3M GuSCN
ISOH 10% 16.7% 25%
Average Ct for Pf 18S rDNA (± SD) 33.9 (± .4) 27.7 (± .2) 29.4 (± .2)
Average Ct for human actin (± SD) 27.0 (± .4) 26.5 (± .2) 26.8 (± .4)
  1. Experiments done with samples at parasite density of 10,000 parasites/mL
  2. Pf, Plasmodium falciparum; GuSCN, guanidine thiocyanate; ISOH, isopropanol; SD, standard deviation; Ct, cycle threshold