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Table 2 Addition of 16.7% isopropanol to the lysis buffer improves recovery of parasite DNA

From: An improved nucleic acid extraction method from dried blood spots for amplification of Plasmodium falciparum kelch13 for detection of artemisinin resistance

 

3M GuSCN

ISOH

10%

16.7%

25%

Average Ct for Pf 18S rDNA (± SD)

33.9 (± .4)

27.7 (± .2)

29.4 (± .2)

Average Ct for human actin (± SD)

27.0 (± .4)

26.5 (± .2)

26.8 (± .4)

  1. Experiments done with samples at parasite density of 10,000 parasites/mL
  2. Pf, Plasmodium falciparum; GuSCN, guanidine thiocyanate; ISOH, isopropanol; SD, standard deviation; Ct, cycle threshold
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Malaria Journal

ISSN: 1475-2875

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