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Table 3 A pH 6.5 or below for the lysis buffer leads to improved recovery of parasite DNA

From: An improved nucleic acid extraction method from dried blood spots for amplification of Plasmodium falciparum kelch13 for detection of artemisinin resistance

pH

7.5

6.5

5.5

Average Ct for Pf 18S rDNA (± SD)

28.6 (± .6)

27.6 (± .1)

27.7 (± .3)

Average Ct for human actin (± SD)

26.2 (± .2)

26.2 (± .2)

26.6 (± .6)

  1. Experiments done with samples at parasite density of 10,000 parasites/mL
  2. Pf, Plasmodium falciparum; GuSCN, guanidine thiocyanate; ISOH, isopropanol; SD, standard deviation; Ct, cycle threshold
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Malaria Journal

ISSN: 1475-2875

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