Table 4 Incubation at 65 °C for 1 h is superior to overnight incubation for recovery of parasite DNA from dried blood spots
Incubation time at 65 °C | 1 h | Overnight | ||
|---|---|---|---|---|
Lysis buffer | 3 M GuSCN | 3 M GuSCN + 16.7% ISOH | 3 M GuSCN | 3 M GuSCN + 16.7% ISOH |
Average Ct for Pf 18S rDNA (± SD) | Undetectable | 30.0 (± .3) | Undetectable | Undetectable |
Average Ct for human actin (± SD) | 29.8 (± .4) | 28.3 (± .3) | 28.4 (± .3) | 26.5 (± .9) |